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目的 探讨铁离子对黑质纹状体多巴胺神经元的毒性作用。方法 采用立体定向偏侧大鼠黑质内注入 5 0 μgFeCl3 和FeCl2 ,4周后用阿朴吗啡诱导动物行为学变化 ,高效液相色谱 (HPLC)检测纹状体内多巴胺、去甲肾上腺素、肾上腺素递质含量的变化 ,免疫组化观察黑质多巴胺神经元和胶质细胞的改变。结果 FeCL3 和FeCL2 均可引起注射侧纹状体内DA含量选择性降低 ,而NA、A含量无显著改变 ;注射侧黑质内DA神经元显著缺失、胶质细胞显著增生 ;FeCL3 组阿朴吗啡诱导大鼠向同侧旋转行为 ,FeCL2 组大鼠于术后即出现特征性自发性对侧旋转行为 ,阿朴吗啡不能诱发其旋转。结论 铁离子对黑质纹状体多巴胺神经元具有毒性作用 ,Fe3 + 作用最强 ,胶质细胞的增生可能参与了这一毒性作用过程。
Objective To investigate the toxic effects of iron on dopamine neurons in nigrostriatal system. Methods We injected 50 μg FeCl3 and FeCl2 into the substantia nigra of stereotaxic rats. After 4 weeks, the morphological changes of the animals were induced by apomorphine. The contents of dopamine, norepinephrine and adrenal gland in the striatum were detected by high performance liquid chromatography (HPLC) Changes in the content of neurotransmitter, immunohistochemical changes of dopamine neurons and glial cells in substantia nigra. Results Both FeCL3 and FeCL2 induced a selective reduction of DA content in striatum, but NA and A contents did not change significantly. DA neurons in substantia nigra were markedly deleted and glial cells were significantly proliferated. Apocalmorph induced by FeCL3 The rats were subjected to ipsilateral rotation behavior, characteristic spontaneous contralateral rotation behavior was observed in FeCL2 rats, apomorphine could not induce their rotation. Conclusion Ferric ions have toxic effects on dopaminergic neurons of nigrostriatal stroma, with the strongest effect of Fe3 +. Glial proliferation may be involved in this toxic effect.