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目的核苷酸切除修复基因XPC(着色性干皮病基因组C)与多种肿瘤的发病相关,通过检测第9内含子PAT-/+和第15外显子A/C单核苷酸多态性(SNP)探讨其与卵巢上皮性癌易感性的关系。方法选取2001年12月至2005年11月在河北医科大学第四医院卵巢上皮性癌患者208例和正常对照者231例。采用聚合酶链反应-限制性片段长度多态性方法检测XPC的多态性基因型。结果XPC基因第9内含子PAT-/+SNP,第15外显子A/CSNP卵巢癌组及对照组的基因型频率分布,等位基因频率两组比较无统计学意义(P>0.05)。以-/-、A/A基因型做参照,与其它两型比较均不能增加卵巢癌的发病风险。将卵巢癌组病理类型分组,分别与正常对照组相比及在各病理类型之间相比较,基因型频率分布无统计学意义(P>0.05)。早期(Ⅰ、Ⅱ期)卵巢癌与晚期(Ⅲ、Ⅳ期)卵巢癌的基因型频率分布无统计学意义(P>0.05)。结论研究未发现XPC基因第9内含子PAT-/+及第15外显子A/C多态与卵巢癌的易感性相关。
The purpose of the nucleotide excision repair gene XPC (Strain Coat X) is related to the pathogenesis of various tumors. By detecting the 9th intron PAT - / + and 15 exon A / C single nucleotide (SNP) to explore its relationship with epithelial ovarian cancer susceptibility. Methods From December 2001 to November 2005, 208 cases of ovarian epithelial cancer in the Fourth Hospital of Hebei Medical University and 231 cases of normal controls were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of XPC genotypes. Results There was no significant difference in genotype frequency and allele frequency between XPC gene intron 9 PAT - / + SNP, exon 15 A / CSNP ovarian cancer and control group (P> 0.05) . With - / -, A / A genotype as a reference, compared with the other two types can not increase the risk of ovarian cancer. The pathological types of ovarian cancer grouping, respectively, compared with the normal control group and in all pathological types, genotype frequency distribution was not statistically significant (P> 0.05). There was no significant difference in the frequency of genotypes distribution between ovarian cancer (stage Ⅰ, Ⅱ) and advanced stage (Ⅲ, Ⅳ) ovarian cancer (P> 0.05). Conclusion The polymorphism of PAT - / + 15 and A / C polymorphism of exon 9 of XPC gene was not associated with susceptibility to ovarian cancer.