确定微小隐孢子虫(C.parum)感染动物模型的虫负荷,以前采用定量和半定量方法。以粪便中的卵囊计数作为对动物肠及肠外器官的组织切片中检查隐孢子虫的补充。传统卵囊计数法在取材与技术上缺乏客观性。本研究提出了用流式细胞仪法对粪便中隐孢子虫卵囊进行定量测定。 The burden of insects on C. parvum infected animal models was determined using quantitative and semi-quantitative methods. The count of oocysts in the stool was used as a supplement to check cryptosporidium in tissue sections of the intestine and parenteral organs. Traditional oocyst counting method in the lack of objectivity and technology. In this study, the quantitative determination of Cryptosporidium oocysts in feces by flow cytometry was proposed.