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为选取具有杀蚜潜力的虫生真菌,采用喷雾法比较研究了玫烟色棒束孢IF-1106和球孢白僵菌BB-1339对桃蚜成蚜的致病力,并利用时间-剂量-死亡率模型估计了2菌株对桃蚜的致死剂量与致死时间。结果表明,菌株IF-1106侵染桃蚜后菌丝如棉絮状,形成淡玫瑰色分生孢子,且菌丝侵染速度快于菌株BB-1339;菌株BB-1339侵染桃蚜后菌丝短而致密,形成白色分生孢子。菌株IF-1106在浓度为1×108CFU/m L时,累计死亡率达到91.7%,而菌株BB-1339最高仅为55.0%。时间-剂量-死亡率模型中Hosmer-Lemeshow方法拟合异质性检验表明模型拟合良好,在接种后7d,菌株IF-1106和BB-1339对桃蚜的半致死浓度分别为1.20×105CFU/m L和8.15×107CFU/m L;当浓度为1×108CFU/m L时,2菌株对桃蚜的半致死时间分别为4.2 d和6.8 d。表明菌株IF-1106对桃蚜的致病力高于菌株BB-1339,更具开发潜力。
In order to select the fungi with the potential to kill aphids, the pathogenicity of the fungus Aphis gossypii to Myzus persicae was compared by spray method. The effects of time-dose - Mortality Model The lethal dose and lethal time of 2 strains of Myzus persicae were estimated. The results showed that mycelium of IF-1106 was infected by Myzus persicae, such as cotton flocculent, forming light rose conidia, and its mycelium infection rate was faster than that of strain BB-1339. After the strain BB-1339 was infected by Myzus persicae, Short and dense, forming white conidia. The strain IF-1106 had a cumulative mortality of 91.7% at a concentration of 1 × 10 8 CFU / m L, while the highest strain of strain BB-1339 was only 55.0%. The heterogeneity test of Hosmer-Lemeshow method in the time-dose-mortality model showed that the model fitted well. The lethal concentrations of IF-1106 and BB-1339 on Myzus persicae were 1.20 × 105CFU / m L and 8.15 × 107CFU / m L, respectively. When the concentration was 1 × 108CFU / m L, the lethal times of 2 strains to Myzus persicae were 4.2 d and 6.8 d, respectively. The results showed that strain IF-1106 had higher developmental potential on Myzus persicae than on strain BB-1339.