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目的:探讨人类三原核合子及二倍体化合子中DNA甲基化模式的变化情况。方法:我们采用显微操作技术去除三原核合子中两个雄原核中的一个,观察恢复了二倍体状态的胚胎的发育情况,并检测了三原核和二倍体化的合子及早期胚胎中DNA甲基化模式的动态变化。结果:二倍体化的合子的囊胚形成率与三原核合子的囊胚形成率无显著性差异;在人三原核合子中两个雄原核发生主动地DNA去甲基化而雌原核在受精后的20h后仍保持甲基化。三原核与二倍体化合子中,DNA甲基化模式没有差别。结论:去除一个雄原核不会影响合子和胚胎的DNA甲基化模式。去除多余雄原核并不能改善胚胎的发育。
OBJECTIVE: To investigate the changes of DNA methylation pattern in human triary and dipyridamole. METHODS: We used microsurgical techniques to remove one of two prokaryotic nuclei from the tripartite nucleosomes and observe the development of diploid-rescued embryos and to detect the presence of prokaryotic and diploidized zygotes and early embryos Dynamic changes of DNA methylation patterns. RESULTS: The blastocyst formation rate of diploidized zygotes did not differ significantly from the blastocyst formation rate of the three-progeny karyon. Two prokaryotic nuclei in human trisomy 3 had active DNA demethylation while the female pronucleus were fertilized After 20h remained methylated. Three prokaryotic and diploid zygotes, DNA methylation patterns no difference. Conclusion: Removing a single pronucleus does not affect DNA methylation patterns in zygotes and embryos. Removal of excess male pronucleus does not improve embryonic development.