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目的探讨复方中药含药血清对人A375黑素瘤细胞酪氨酸酶(TYR)活性的影响。方法体外培养人A375黑素瘤细胞,采用多巴速率氧化法研究复方中药含药血清对TYR活性的影响。结果低浓度(5%～10%)的Ⅰ方、Ⅱ方、Ⅲ方对酪氨酸酶活性无明显激活作用(P>0.05);高浓度(20%～30%)的Ⅰ方、Ⅲ方、Ⅵ方对TYR活性均有明显激活作用(P<0.05),以Ⅵ方作用最显著,且与其它各组相比,差异均有统计学意义(P<0.01)。结论对TYR具有激活作用的中药组成复方并不一定对TYR有激活作用,具有祛风活血和补肾三重作用的Ⅵ方在促进TYR活性上优于祛风除湿Ⅰ方、养血活血Ⅱ方和滋补肝肾Ⅲ方。
Objective To investigate the effect of compound Chinese medicine serum on the tyrosinase (TYR) activity of human A375 melanoma cells. Methods Human A375 melanoma cells were cultured in vitro. The effect of compound Chinese medicine serum on TYR activity was studied by the dopa rate oxidation method. Results No significant activation of tyrosinase activity was observed at low concentration (5% ~ 10%) of side Ⅰ, side Ⅱ, and side Ⅲ (P> 0.05). In high concentration (20% ~ 30% (P <0.05). The effect of Ⅵ was the most significant, and the difference was statistically significant compared with other groups (P <0.01). Conclusion TYR has the activating effect of traditional Chinese medicine compound does not necessarily have a role in the activation of TYR, with Qufeng Huoxue and kidney three-party role in promoting TYR activity is superior to expelling wind and dampness Ⅰ, nourishing blood and activating blood Ⅱ side and nourishing Liver and kidney III side.