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本研究利用48个SSR标记将12个不育系(保持系)、8个恢复系划分为4个组群,并利用NCⅡ设计共配制96个组合,研究不同组群亲本配组的杂种优势表现。研究结果表明,不育系和恢复系分别被分为3和2个组群,共产生6种配组模式,两系主栽不育系×扬稻6号类群恢复系(G2×G3)、三系保持系×三系恢复系(G4×G1)、扬稻6号类群不育系×三系恢复系(G3×G1)三类配组模式杂种优势明显。进一步研究显示遗传距离与杂种优势之间相关不显著。生育期是三系恢复系与两系主栽不育系配组(G2×G1)杂种低产的限制因子。扬稻6号类群不育系×三系恢复系(G3×G1)是一种新的杂种优势模式,因此,筛选、培育与扬稻6号属于同一类群的新型不育系,可进一步拓宽现有三系恢复系的利用范围,提高籼型杂种优势利用水平。
In this study, 48 SSR markers were used to divide 12 CMS lines and 8 restorer lines into 4 groups. A total of 96 combinations were designed by NCⅡ design to study the heterosis performance of parents . The results showed that the CMS lines and restorer lines were divided into 3 groups and 2 groups, respectively. There were 6 types of mating patterns, including two CMS lines × G2 × G3, Three types of maintainer lines × three line restorer lines (G4 × G1), Yangdao 6 × CMS lines (G3 × G1) three types of matching group hybrids obvious advantages. Further studies showed no significant correlation between genetic distance and heterosis. The growth period is the limiting factor for the low yield of hybrids between three-line restorer lines and two-line main CMS lines (G2 × G1). Therefore, screening and breeding of a new CMS line belonging to the same taxa of Yangdao 6 could further widen the scope of current CMS lines (G3 × G1) There are three lines restore the use of the Department, improve the utilization of indica hybrids.