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目的 探讨食管癌端粒酶活性检测的临床意义。方法 建立并应用ＰＣＲ 技术为基础的端粒重复序列扩增，对３６ 例食管癌及其癌旁组织进行检测。结果 ３６ 例食管癌组织中２９ 例端粒酶活性呈阳性，阳性检出率为８０ ．５０ ％ ，癌旁组织中２ 例呈阳性，阳性检出率为６．００％ ；二者比较差异有非常显著性（χ２ ＝ ４１．３０，Ｐ＜０ ．００１）；３６ 例食管癌均为鳞状细胞癌，其中１７ 例伴随淋巴结转移的标本中端粒酶活性均呈阳性，而在１９ 例未伴随淋巴结转移的病人中仅有１２ 例检出端粒酶活性，其活性检出率为６３．１５％ ，二者比较差异有非常显著性（ Ｐ＝ ０ ．００６）。结论 端粒酶活性的检测可作为食管癌诊断的指标之一，同时对判断食管癌的预后具有重要参考价值。
Objective To explore the clinical significance of detection of telomerase activity in esophageal cancer. Methods Thirty-six repeats of telomere repeats based on PCR were established and applied to detect 36 cases of esophageal cancer and adjacent tissues. Results The telomerase activity was positive in 29 cases of esophageal cancer. The positive detection rate was 80. 50% of the cancer tissues were positive in 2 cases and the positive detection rate was 6.00%. There was a significant difference between the two groups (χ2=41.30, P<0.001); 36 cases of esophageal cancer were In squamous cell carcinoma, telomerase activity was positive in 17 specimens with lymph node metastasis, while telomerase activity was detected in only 19 specimens without lymph node metastasis. The activity detection rate was 63.15%, the difference between the two is very significant (P = 0. 006). Conclusion The detection of telomerase activity can be used as one of the indicators for the diagnosis of esophageal cancer, and it is also an important reference for judging the prognosis of esophageal cancer.