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目的 建立垂体瘤组织基因表达谱并探讨其发病的分子机理。方法垂体生长激素瘤（ＧＨＰＡ）和无功能腺瘤（ＮＦＰＴ）ｃＤＮＡ文库构建，大规模表达序列标签（ｅｘｐｒｅｓｓｅｄ ｓｅｑｕｅｎｃｅ ｔａｇｓ，ＥＳＴｓ）测序及生物信息学处理。结果 在已知基因中，参与基因蛋白表达者最高（分别为２９％和２８％），其次是代谢相关基因（分别为１８％和２１％），均较正常垂体为高（２３．２％和１４．３％）。ＧＨＰＡ中可同时表达泌乳素（ＰＲＬ）和生长激素（ＧＨ）；ＧＨＰＡ和正常垂体（ＮＰ）中ＧＳａ基因表达明显高于钙调素，而ＮＦＰＴ中则相反。在垂体瘤差异表达的与细胞分裂有关的基因中，有些与肿瘤的发生有关；发现了７４个在垂体瘤中高表达而正常垂体中不表达的未知基因的ＥＳＴｓ。结论 不同垂体瘤组织中其主要的信号转导途径可能有别；发现有些与细胞分裂有关的基因在ＮＦＰＴ或ＧＨＰＡ库中与ＮＰ库中表达不同。
Objective To establish the gene expression profile of pituitary tumors and explore the molecular mechanism of its pathogenesis. Methods Pituitary growth hormone tumor (GHPA) and non-functioning adenoma (NFPT) cDNA library construction, sequencing of large expressed sequence tags (ESTs), and bioinformatics processing were performed. Results Among the known genes, those involved in gene protein expression were the highest (29% and 28%, respectively), followed by metabolism-related genes (18% and 21%, respectively), which were higher than the normal pituitary (23.2% and 14.3%). Prolactin (PRL) and growth hormone (GH) can be simultaneously expressed in GHPA; the expression of GSa gene in GHPA and normal pituitary (NP) is significantly higher than that in calmodulin, whereas it is the opposite in NFPT. Among the genes associated with cell division that are differentially expressed in pituitary tumors, some are related to the occurrence of tumors; 74 ESTs that are highly expressed in pituitary tumors but not in normal pituitary are found. Conclusions The major signal transduction pathways may be different in different pituitary tumors; some genes related to cell division are found to be different in NFPT or GHPA pools from NP pools.