原位杂交(ISH)可显示细胞内mRNA的存在和数量,由于它能研究单个细胞及细胞分化而在血液学中特别受到重视,它可以用于组织切片或细胞培养,在标本量少,或表达的DNA和mRNA的拷贝数很少的情况下显示出它的极大价值。在血液中ISH提供有兴趣的信息是:Koury氏等指出红细胞生成素(Epo)是由肾曲管基底膜以外的细胞产生,Lacombc氏等证实合成Epo的细胞同时与抗VⅢ因子相关抗原起反应而认为是内皮细胞。 In situ hybridization (ISH) shows the presence and quantity of intracellular mRNAs, which are of particular interest in hematology due to its ability to study individual cells and cell differentiation. It can be used in tissue sections or cell culture where the amount of specimen is small, or It shows great value in the case of few copies of expressed DNA and mRNA. Interesting information provided by ISH in the bloodstream is that Koury et al. Indicate that erythropoietin (Epo) is produced by cells other than the renal tubule basement membrane and that Lacombc et al. Demonstrated that cells that synthesize Epo react with anti-VIII-related antigens And that is endothelial cells.