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目的:探讨槲皮素(Que)对高糖培养海马神经元的保护作用及其作用机制。方法:原代培养新生大鼠海马神经元3d,随机分为正常对照组、高糖组、阳性对照组及Que 2.5μmol/L(Q2.5)、Que 5μmol/L(Q5)、Que 7.5μmol/L(Q7.5)组,不同条件培养72h后,用流式细胞仪检测神经元凋亡、Western Blot检测GRP78、Caspase-12的表达。结果:与正常对照组比较,高糖组、Que三组及阳性对照组的神经元凋亡率及GRP78、Caspase-12表达均显著增加(P<0.01)。与高糖组比较,阳性对照组和Que各组神经元凋亡率均显著下降(P<0.01,P<0.05);Que各组的GRP78和Caspase-12的表达显著下降(P<0.01),其中Q5和Q7.5组下降尤为显著,且两组比较无统计学差异;与阳性对照组比较,Q5和Q7.5组的神经元凋亡率和抑制Caspase-12表达的作用均无显著性差异。结论:槲皮素能够减轻高糖培养海马神经元凋亡,在此情况下,GRP78、Caspase-12表达均下降,提示槲皮素抑制海马神经元凋亡的作用机制可能与内质网应激有关。
Objective: To investigate the protective effect of quercetin on hippocampal neurons cultured in high glucose and its mechanism. Methods: Primary cultured neonatal rat hippocampal neurons were randomly divided into 3 groups: normal control group, high glucose group, positive control group and Que 2.5μmol / L (Q2.5), Que 5μmol / L /L(Q7.5) group. After 72 hours of culture, neuronal apoptosis was detected by flow cytometry. The expression of GRP78 and Caspase-12 was detected by Western Blot. Results: Compared with the normal control group, the apoptotic rate of neurons and the expressions of GRP78 and Caspase-12 in high glucose group, Que group and positive control group were significantly increased (P <0.01). Compared with the high glucose group, the apoptotic rate of neurons in the positive control group and the Que groups were significantly decreased (P <0.01, P <0.05); the expression of GRP78 and Caspase-12 in the Que groups was significantly decreased (P <0.01) The Q5 and Q7.5 groups were significantly decreased, and there was no significant difference between the two groups; compared with the positive control group, there was no significant difference between the Q5 and Q7.5 groups in the apoptotic rate and the inhibition of Caspase-12 expression difference. CONCLUSION: Quercetin can reduce the apoptosis of hippocampal neurons cultured in high glucose, and the expression of GRP78 and Caspase-12 is decreased in this condition, suggesting that quercetin may inhibit the apoptosis of hippocampal neurons and its mechanism may be related to endoplasmic reticulum stress related.