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作者调查了幽门螺杆菌(HP)的细胞毒素在正常人粘膜原代上皮细胞中引起空泡的活性。正常人粘膜上皮细胞来自空肠组织,经酶消化和筛选提纯。在接种HP培养物上清液前后,都用光镜和电镜进行空泡测定。接种对HeLa细胞具有形成空泡活性(Tox~+)的HP60190株(ATCC49503),与缺乏空泡形成活性(Tox~-)的同源性突变株60190-VL株,并同时用肉汤作为对照。培养结果用光镜检测比较,发现暴露在Tox~+菌的培养物上清液中的原代上皮细胞(纯度>98%),有52%±2%细胞出现一个或多个空泡;暴露在Tox~-菌培养物上清液中仅23%±2.3%细胞出现空泡;在对照肉汤中,上皮细胞出现空泡为23%±3.7%(P<0.05)。在抗体抑制试验中,作者用特异性免疫
The authors investigated the vacuolar activity of Helicobacter pylori (HP) cytotoxins in normal human mucosal primary epithelial cells. Normal human mucosal epithelial cells from the jejunal tissue, digested by enzymes and screening and purification. Before and after the inoculation of HP culture supernatant, the vacuoles were measured by light microscope and electron microscope. HP60190 strain (ATCC49503) having vacuolar activity (Tox ~ +) to HeLa cells was inoculated with the same strain 60190-VL strain lacking vacuolar forming activity (Tox ~ -), and broth was used as a control . The results of the culturing showed that one or more vacuoles appeared in 52% ± 2% of cells exposed to primary epithelial cells (purity> 98%) in culture supernatants of Tox + Only 23% ± 2.3% of the cells in the Tox-bacteria culture supernatant showed vacuoles; in control broth, the vacuolation of epithelial cells was 23% ± 3.7% (P <0.05). In antibody inhibition tests, the authors used specific immunity