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人胚鼻咽粘膜移植于裸鼠皮下后可长期存活并保持其原有组织结构和功能,在此基础上用二亚硝基哌嗪皮下给药法诱发其癌变。在40周内,低剂量多次给药组(12例)见不典型增生6例次,原位癌1例,早期浸润癌1例;一次性给药组(11例)见不典型增生5例次,原位癌3例。用抗P_(21)单抗检测rasP_(21)蛋白表达,发现异型增生的基底细胞较正常基底细胞表达增强,可作为癌前病变的指标之一。
Human embryonic nasopharyngeal mucosa can survive for a long period of time after transplanted into the skin of nude mice and maintain its original tissue structure and function. On this basis, subcutaneous administration of dinitrosopiperazine induces its carcinogenesis. Within 40 weeks, low-dose multiple dosing group (12 cases) saw 6 cases of atypical hyperplasia, 1 case of carcinoma in situ, and 1 case of early invasive carcinoma; 1 case of single-dose administration group (11 cases) saw atypical hyperplasia 5 Cases, 3 cases of carcinoma in situ. Anti-P_(21) monoclonal antibody was used to detect the expression of rasP_(21) protein. It was found that the dysplasia of basal cells increased compared with normal basal cells and could be used as one of the indicators of precancerous lesions.