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目的探讨鸡矢藤苷酸甲酯在小鼠疼痛模型中的活性研究。方法在2013年4—9月将170例小鼠随机分为17组,每组10只。首先从植物鸡矢藤中分离提取出鸡矢藤苷酸甲酯。将鸡矢藤苷酸甲酯分别按照不同的剂量注射在受试小鼠,在小鼠醋酸扭体实验,小鼠福尔马林实验和小鼠热板实验中进行其对化学和热刺激引发的疼痛的耐受性测试,分析鸡矢藤苷酸甲酯的镇痛活性。计量资料采用t检验,P<0.05为差异具有统计学意义。结果小鼠醋酸扭体的外周镇痛实验的结果显示:鸡矢藤苷酸甲酯3、4、5组对腹腔注射醋酸引发的小鼠扭体次数[(26.8±4.6)、(26.3±3.4)、(22.5±3.0)次]与空白组[(44.2±4.7)次]相比有显著的抑制作用,差异均有统计学意义(均P<0.05);小鼠热板的中枢镇痛实验显示:鸡矢藤苷酸甲酯4、5组对热刺激的耐受时间[(2.45±0.30)、(2.46±0.24)s]较空白组[(1.52±0.15)s]具有明显的延长,差异均有统计学意义(均P<0.05)。而小鼠福尔马林实验中,鸡矢藤苷酸甲酯组在0~5min与25~30min的时间段内有良好的外周和中枢神经镇痛效果[(36.40±4.80)、(5.40±2.66)次],与空白组[(83.83±13.56)、(45.17±12.86)次]相比差异均有统计学意义(均P<0.05)。结论鸡矢藤苷酸甲酯对小鼠疼痛模型具有中枢和外周双重镇痛调节作用,提示其作为疼痛治疗或者预防药物具有一定的开发价值。
Objective To investigate the activity of methyl guaifenesin in a mouse model of pain. Methods From April to September in 2013, 170 mice were randomly divided into 17 groups with 10 mice in each group. First, the plant chicken rotiflorin isolated and extracted methyl guanosine methyl ester. The chicken tilapia methyl ester was injected into the mice at different doses, respectively, and was subjected to chemical and thermal stimulation in mouse acetic acid writhing test, mouse formalin test and mouse hot plate test Pain Tolerance Test Analgesic activity of chicken rotolide methyl ester was analyzed. Measurement data using t test, P <0.05 for the difference was statistically significant. Results The results of the peripheral analgesia experiment of acetic acid writhing in mice showed that the number of writhing induced by intraperitoneal injection of acetic acid in mice in group 3,4 and 5 [(26.8 ± 4.6), (26.3 ± 3.4) , (22.5 ± 3.0) times compared with the blank group [(44.2 ± 4.7) times], the differences were statistically significant (all P <0.05). The central analgesic experiment on the hot plate of mice showed : Compared with blank group [(1.52 ± 0.15) s], the tolerance time of chicken tilapia methyl 4,5 groups to thermal stimulation was significantly (2.45 ± 0.30) and (2.46 ± 0.24) s, respectively, There was statistical significance (all P <0.05). However, in the formalin test of mice, there was good peripheral and central analgesic effects in the methylgalatoprostate group during 0-5 min and 25-30 min [(36.40 ± 4.80), (5.40 ± 2.66 ) Times] compared with those in the blank group [(83.83 ± 13.56) and (45.17 ± 12.86) times], respectively (all P <0.05). Conclusion Chicken tilapia methyl ester has dual central and peripheral analgesic effects on the pain model in mice, suggesting that it may be of value in the development of pain management and prophylaxis.