A series of studies were conducted to establish a methodology for the accurate and efficient determination of can-thaxanthin in feed ingredients.Agilent ZORBAX Eclipse DB-C18 analytical column(4.6×150 mm,5 μm) was used and kept at 25℃.The mobile phase was acetonitrile:methanol(v/v)=95 :5,and eluted compounds were detected by DAD absorbance(470 nm).The flow rate was maintained at 1.0 mL · min-1.The response of the method was linear over the range 1.0-20.0 μg· mL-1 canthaxanthin assay solution(R2=0.9998).Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%.Variation coefficient was less than 3.53%.This method is proved to be simple,precise,sensitive and reproductive. A series of studies were conducted to establish a methodology for the accurate and efficient determination of can-thaxanthin in feed ingredients. Agilent ZORBAX Eclipse DB-C18 analytical column (4.6 × 150 mm, 5 μm) was used and kept at 25 ° C. The mobile phase was acetonitrile: methanol (v / v) = 95: 5 and eluted compounds were detected by DAD absorbance (470 nm). The flow rate was maintained at 1.0 mL · min -1 the range of 1.0-20.0 μg · mL -1 canthaxanthin assay solution (R2 = 0.9998) .Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103% .Variation coefficient was less than 3.53%. This method is proved to be simple, precise, sensitive and reproductive