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为筛选出糯性小麦材料,研究糯性小麦籽粒的出现频率,明确各分离群体的Wx基因型,使筛选的糯性与非糯性材料呈现出表现型与基因型的统一,以生产上大面积推广应用的普通小麦品种克旱16、克春5号为母本,糯性小麦品种宁糯1号、南农09P18为父本4个杂交组合的F2代分离群体为研究对象,以半籽粒碘染色与分子检测法为研究手段,对F2分离群体进行糯与非糯性材料鉴定与筛选。结果表明:组合克旱16/宁糯1号、克春5号/宁糯1号、克旱16/南农09P18、克春5号/南农09P18糯性小麦的出现频率分别为1.1%、1.4%、1.4%、1.3%;从4个组合的F2群体100个单株中筛选到了共11株Waxy基因全缺型的植株,与碘染色结果一致。
In order to screen out the waxy wheat material, study the occurrence frequency of waxy wheat grain, clarify the Wx genotype of each segregation population, make the selected waxy and non-waxy material show the unity of phenotype and genotype, The F2 popula- tions of common wheat cultivars Kailu 16 and Kechun 5 as female parent and Waxy wheat cultivar Ningyou 1 and Nannong 09P18 as their male parents were used as research objects. Iodine staining and molecular detection as research methods, identification and selection of waxy and non-waxy materials for F2 segregation population. The results showed that the frequency of waxy wheat combination Kudan 16 / Ning Nuo 1, Kechun 5 / Ning Nuo 1, Khan 16 / Nannong 09P18, Kechun 5 / Nannong 09P18 were 1.1% 1.4%, 1.4% and 1.3% respectively. A total of eleven Waxy gene-deficient plants were screened from 100 F2 plants in four combinations, which was consistent with the result of iodine staining.