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用前期筛选的芽孢杆菌5a、6a菌株和酵母菌Y-1、Y-2、Y-2-0菌株经6次紫外线诱变,共获得21株对芒果炭疽病菌抑菌作用增强的诱变菌株。其中芽孢杆菌诱变菌株5a-9、6a-3、6c-4、6c-5、酵母菌诱变菌株Kck、M2抑菌圈直径依次增大了41.0%、54.7%、53.3%、45.3%、44.1%和40.7%,诱变效应明显;耐药性筛选结果表明,质量浓度为2μg/mL多菌灵和咪鲜胺锰盐可与诱变后所得的各菌株间形成优良组合。在芒果果实上抑菌效果表明,大部分生防菌与药剂间具有协同作用。其中多菌灵和咪鲜胺锰盐分别与芽孢杆菌诱变菌株5a-9、6a-3、酵母菌诱变菌株M2、H1组合,抑菌效果达63.4%～73.5%,比两种杀菌剂单用的抑菌效果提高了8.3%～18.4%。
A total of 21 strains of mutants with enhanced bacteriostasis against Colletotrichum gloeosporioides were obtained by 6 UV mutagenesis of the previously screened Bacillus 5a and 6a and Y-1, Y-2 and Y-2-0 strains. . The diameters of bacteriostatic circles of Bacillus mutagenesis strains 5a-9,6a-3,6c-4,6c-5 and yeast mutant strains Kck and M2 increased by 41.0%, 54.7%, 53.3% and 45.3%, respectively, 44.1% and 40.7%, respectively. The mutagenic effect was obvious. The results of drug resistance screening showed that the combination of carbendazim and prochloraz at the concentration of 2μg / mL could form an excellent combination with the mutants. Antibacterial effect on the mango fruit shows that most of the biocontrol bacteria and synergies between drugs. Among them, carbendazim and prochloraz were combined with Bacillus mutants 5a-9,6a-3 and yeast mutagens M2 and H1, respectively. The antibacterial effect of carbendazim and prochloraz was 63.4% -73.5% Antibacterial effect alone increased by 8.3% to 18.4%.