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目的探讨通塞颗粒对大鼠慢性阻塞性肺疾病(COPD)急性加重期模型炎症细胞的影响。方法Wistar大鼠分为7组:对照组、COPD稳定期模型组、COPD急性加重期模型组、通塞颗粒高剂量组、通塞颗粒低剂量组、氨茶碱组、葶贝胶囊组,各10只。以反复呼吸道细菌感染的方法建立COPD急性加重期模型。对照组和模型组给以灌胃生理盐水,每日2次。通塞颗粒高、低剂量组、氨茶碱组、葶贝胶囊组分别给以中药通塞颗粒5g/(kg.d)、中药通塞颗粒2.5g/(kg.d)、氨茶碱2.3mg/(kg.d)、中药葶贝胶囊0.476g/(kg.d)灌胃,每日2次,给药时间为1周。观察通塞颗粒对外周血、支气管肺泡灌洗液中炎症细胞、肺功能的影响。结果外周血白细胞、中性粒细胞计数显示急性加重期模型组高于空白组和稳定期模型组(P<0.01,P<0.05);高剂量组低于氨茶碱组(P<0.05),外周血中性粒细胞计数显示高剂量组低于葶贝胶囊组(P<0.05)。高、低剂量组明显低于急性加重期模型组(P<0.01,P<0.05);白细胞和中性粒细胞计数显示稳定期模型组支气管肺泡灌洗液中高于空白对照组(P<0.05,P<0.01)、低于急性加重期模型组(P<0.01);高剂量组、低剂量组明显低于急性加重期模型组(P<0.05,P<0.01)和葶贝胶囊组(P<0.01,P<0.05);高剂量组支气管肺泡灌洗液中中性粒细胞明显低于氨茶碱组(P<0.01)。造模各组大鼠潮气量(Vt)、分钟通气量(MVV)、FEV0.3/FVC和呼气峰流速(PEF)明显低于空白对照组(P<0.01);急性加重期模型组、通塞颗粒低剂量组、葶贝胶囊组、氨茶碱组Vt、MVV、FEV0.3/FVC%和PEF均明显低于稳定期模型组(P<0.01)。通塞颗粒高剂量组、低剂量组、氨茶碱组Vt明显高于急性加重期模型组(P<0.05),通塞颗粒高剂量组MVV、FEV0.3/FVC和PEF均明显高于急性加重期模型组(P<0.05),吸气阻力和呼气阻力均低于急性加重期模型组(P<0.05);通塞颗粒高剂量组Vt、MVV明显高于葶贝胶囊组(P<0.05),吸气阻力和呼气阻力明显低于葶贝胶囊组(P<0.05)。结论通塞颗粒有一定抑制白细胞和中性粒细胞趋化和抗感染作用,能够改善COPD急性加重期模型组的肺通气功能,其作用明显优于中药葶贝胶囊。
Objective To investigate the effect of Tongsai granule on inflammatory cells in a rat model of chronic obstructive pulmonary disease (COPD) exacerbation. Methods Wistar rats were divided into 7 groups: control group, COPD stable model group, COPD acute exacerbation model group, Tongsai Granule high-dose group, Tongsai Granule low-dose group, aminophylline group, 10 only Acute exacerbation model of COPD was established by recurrent respiratory tract bacterial infection. Control group and model group were given normal saline, 2 times a day. Tongshen granule high and low dose group, aminophylline group and Baobei capsule group were treated with Tongzong Granule 5g / (kg.d), Tongsheng Granule 2.5g / (kg.d), Aminophylline 2.3 mg / (kg · d), the Chinese medicine Beibei capsule 0.476g / (kg.d) intragastric administration, 2 times a day, the administration time is 1 week. To observe the effect of Tongsai granule on the inflammatory cells and lung function in peripheral blood and bronchoalveolar lavage fluid. Results The peripheral blood leucocyte and neutrophil count showed that the acute exacerbation model group was higher than the blank group and the stable model group (P <0.01, P <0.05), the high dose group was lower than the aminophylline group (P <0.05) Peripheral blood neutrophil count showed that the high-dose group was lower than the control group (P <0.05). (P <0.01, P <0.05). The counts of leukocytes and neutrophils in bronchoalveolar lavage fluid of model group were higher than that of blank control group (P <0.05, P < (P <0.01), lower than the acute exacerbation model group (P <0.01); high dose group and low dose group were significantly lower than the acute exacerbation model group (P <0.05, P <0.01) 0.01, P <0.05); neutrophil in bronchoalveolar lavage fluid of high dose group was significantly lower than that of aminophylline group (P <0.01). Compared with the blank control group, the tidal volume (Vt), minute ventilation (MVV), FEV0.3 / FVC and expiratory peak flow velocity (PEF) in the model group were significantly lower than those in the blank control group Vt, MVV, FEV0.3 / FVC% and PEF in Tongsai Granules low dose group, Guibei capsule group and aminophylline group were significantly lower than those in the stable model group (P <0.01). The Vt of Tongsai Granules high dose group, low dose group and aminophylline group was significantly higher than that of acute exacerbation model group (P <0.05). The MVV, FEV0.3 / FVC and PEF of Tongsai Granule high dose group were significantly higher than those of acute group (P <0.05), and the inspiratory resistance and expiratory resistance were lower than those in acute exacerbation model group (P <0.05). The Vt and MVV of Tongsai Granule high dose group were significantly higher than those of Rhubarb capsule group (P < 0.05). The inspiratory resistance and expiratory resistance were significantly lower than those of the control group (P <0.05). Conclusion Tongsai Granule can inhibit leukocyte and neutrophil chemotaxis and anti-infective effect, and can improve lung ventilation function of acute exacerbation COPD model group, and its effect is obviously better than that of Chinese medicine Bayi capsule.