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目的了解异种移植人卵巢组织病理学的变化。方法无菌条件下,随机将冻融后的人卵巢组织细片移植到不同性别的小鼠皮下,按移植后1周、4周、8周、10周的时间分别处死小鼠,收集移植后人卵巢组织,以解冻后移植前的人卵巢组织作对照,所有组织常规脱水包埋制片,HE染色,镜下观察,比较移植后的人卵巢组织片的卵泡存活、生长、凋亡以及间质重构等情况。结果移植1周后,有大量的新生血管满布于人卵巢组织间质并穿插蔓延,无规律性,与人卵巢组织自然生理血管分布有明显的差别;小鼠性别对移植1周的人卵巢组织中原始卵泡无影响。随着移植时间的推移,卵巢组织间质中的血管逐渐减少,与人卵巢组织正常生理血管分布相似;生长卵泡明显增加。结论人卵巢组织可以移植在不同性别小鼠身上并成活;移植后人卵巢组织的闭锁卵泡及生长卵泡都增加,能产生窦卵泡。
Objective To investigate the histopathological changes of ovarian tissue in xenotransplantation. Methods Aseptically frozen human ovarian tissue fragments were transplanted subcutaneously into mice of different sexes. The mice were sacrificed at 1 week, 4 weeks, 8 weeks and 10 weeks after transplantation, respectively. After transplantation, Human ovarian tissue was taken as control before thawing and after transplantation. All tissues were routinely dehydrated and embedded, HE staining and microscopic observation. The survival, growth and apoptosis of follicles in human ovarian tissue after transplantation were compared. Quality restructuring and so on. Results One week after transplantation, a large number of new blood vessels were over-covered in interstitial tissue of human ovarian tissue and interspersed with interspersed irregularities. There was a significant difference between them and the distribution of natural physiological blood vessels in human ovarian tissues. No effect of primordial follicles in the organization. With the passage of time, the blood vessels in the interstitial ovarian gradually reduced, similar to the normal physiological blood vessels of human ovarian tissue distribution; growth follicles significantly increased. Conclusion Human ovarian tissue can be transplanted in different sex mice and survive; allograft ovarian follicles and growth follicles are increased after transplantation, which can produce antral follicles.