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目的 了解外源性肺表面活性物质 (PS)对小肠缺血再灌注所致肺损伤的治疗效果。方法 正常雄性SD大鼠 2 7只 ,腹腔麻醉后随机分为 3组 (n =9) ,A组 :钳夹肠系膜上动脉 10s作为对照 ;B组 :阻断动脉 1h后 ,去除阻断 ,形成再灌注 2h ;C组 :模型制作同B组 ,PS以 10 0mg/kg气道滴入。所有动物再机械通气 2h。监测动脉血气 (PaO2 )、肺顺应性 (Cdyn)和气道阻力 (R)和病理改变。结果 血气分析B组PaO2 进行性降低 (B组与A组比较 ,P <0 .0 5 ) ,C组PS治疗后PaO2 较为稳定 ,不表现为进行性下降 ,12 0min时较病变组差异有显著性意义 (C组与B组比较 ,P <0 .0 5 ) ,肺功能表现为B组Cdyn降低、R升高 ,治疗后Cdyn稳定 ,12 0min时略有升高 (C组与B组比较 ,P <0 .0 5 ) ,病理观察 :B组肺水肿、间质出血、炎性细胞浸润及部分肺不张 ,C组治疗后病变减轻。结论 外源性肺表面活性物质在这种模型的肺损伤治疗中可明显改善肺功能
Objective To investigate the therapeutic effect of exogenous pulmonary surfactant (PS) on lung injury induced by intestinal ischemia-reperfusion. Methods Twenty-seven male Sprague-Dawley rats were randomly divided into 3 groups (n = 9) undergoing abdominal anesthesia. Group A: 10s of superior mesenteric artery as control; Group B: Reperfusion 2h; Group C: model with the same group B, PS to 10mg / kg airway instillation. All animals and then mechanical ventilation 2h. PaO2, lung compliance (Cdyn) and airway resistance (R) and pathological changes were monitored. Results Blood gas analysis PaO2 decreased progressively in group B (group B compared with group A, P <0. 05), group Pa was more stable after PS treatment in group C, did not show progressive decline, and the difference was significant at 120 min (P <0. 05 in group C and group B). The lung function showed that Cdyn decreased and R increased in group B, Cdyn was stable after treatment and slightly increased at 120 min , P <0.05). Pathological observation: Pulmonary edema, interstitial hemorrhage, inflammatory cell infiltration and some atelectasis in group B were alleviated. Conclusions Exogenous pulmonary surfactant can significantly improve pulmonary function in the treatment of lung injury in this model