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目的:建立RP-HPLC同时测定管花肉苁蓉药材中松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷含量的方法。方法:采用Megres-C18(250mm×4.6mm,5μm)色谱柱,柱温30℃;流动相为甲醇(A)-0.1%甲酸(B),梯度洗脱(0min,30%A;30min,40%A;40min,45%A),流速1.0mL·min-1;检测波长330nm。结果:在上述色谱条件下,松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷获得良好分离;进样量分别在0.732~11.7μg(r=0.9991)、0.216~3.45μg(r=0.9995)、0.209~3.35μg(r=0.9991)、0.151~2.42μg(r=0.9993)范围内呈良好的线性关系;平均加样回收率(n=9)分别为101.8%,98.8%,101.1%,99.9%;精密度试验的RSD小于0.65%;重复性试验的RSD小于2.4%。结论:该方法简便、准确,重复性好,为管花肉苁蓉药材的质量控制提供可借鉴的分析方法。
Objective: To establish a method for the simultaneous determination of echinacoside, anguillin A, verbascoside and isogamidine in Cistanche tubulosa by RP-HPLC. METHODS: Megres-C18 (250 mm × 4.6 mm, 5 μm) column was used and the column temperature was 30 ℃. The mobile phase consisted of methanol (A) -0.1% formic acid % A; 40min, 45% A), flow rate 1.0mL · min-1; detection wavelength 330nm. Results: Echinacoside, anguillin A, verbascoside and isogamidoside were well separated under the above chromatographic conditions. The injection volumes were 0.732 ~ 11.7μg (r = 0.9991), 0.216 ~ 3.45μg (r = 0.9995), 0.209 ~ 3.35μg (r = 0.9991), 0.151 ~ 2.42μg (r = 0.9993). The average recoveries were 101.8%, 98.8% and 101.1% , 99.9%; RSD of precision test is less than 0.65%; RSD of repeatability test is less than 2.4%. Conclusion: The method is simple, accurate and reproducible. It provides a reference for the quality control of Cistanche tubulosa.