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目的探讨提取文殊兰Crinum asiaticum种子中总生物碱的最佳提取方法以及工艺条件。方法以文殊兰种子为原料,采用超声和加热回流2种提取方法提取文殊兰种子中的总生物碱。结果响应曲面法优化结果表明,超声波法超声时间47 min,超声温度58℃,超声功率600 W,乙醇体积分数为95%,料液比1∶6,提取3次,提取总生物碱得率0.435 7%;加热回流法提取时间2.5 h,提取温度84℃,料液比1∶9,乙醇体积分数为95%,提取3次,提取总生物碱得率0.449 4%。结论综合考虑总生物碱提取率、操作方法、除杂难易程度等因素,选用加热回流提取文殊兰种子中总生物碱较理想。
Objective To explore the optimum extraction method and process conditions of total alkaloids extracted from Crinum asiaticum seeds. Methods The seeds of Manjusland were used as raw materials, and the total alkaloids in seeds of Manjusvarius were extracted by ultrasonic and heat refluxing methods. Results The response surface methodology showed that the total yield of alkaloid was 0.435, the ultrasonic time was 47 min, the ultrasonic temperature was 58 ℃, the ultrasonic power was 600 W, the volume fraction of ethanol was 95%, the ratio of solid to liquid was 1: 6, 7%; heating reflux method extraction time 2.5 h, extraction temperature 84 ℃, solid-liquid ratio of 1: 9, ethanol volume fraction of 95%, extracted three times, total alkaloid extraction rate of 0.449 4%. Conclusion Considering the total alkaloid extraction rate, operation methods and the ease of impurity removal, the total alkaloids extracted from the seeds of Wenchuanlan by heating and refluxing are more ideal.