论文部分内容阅读
为探讨镉－金属硫蛋白（Ｃｄ－ＭＴ）是否可诱发肾脏细胞的凋亡，首先每天给７周龄雄性Ｗｉｓｔａｒ大鼠皮下注射２ｍｇ／ｋｇＣｄＣｌ２。２周后处死动物，取出肝脏，制备匀浆。１０５０００ｇ离心后取上清液，用ＳｅｐｈａｄｅｘＧ－７５色谱和ＤＥＡＥＡ－２５阳离子交换色?
To investigate whether cadmium-metallothionein (Cd-MT) could induce apoptosis of kidney cells, male Wistar rats were injected subcutaneously with 2mg / kg CdCl2 twice a day for 2 weeks. Animals were sacrificed and the liver was removed to prepare homogenate. After centrifugation, the supernatant was taken at 105000g, and the color was analyzed by Sephadex G-75 and DEAEA-25 cation exchange chromatography.