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2013年对低温胁迫下烟苗活性氧代谢特性以及活性氧与烟草花芽分化关系进行了研究。结果表明,低温胁迫下,保护酶超氧化物歧化酶(SOD)响应最为迅速,随后过氧化氢酶(CAT)活性逐渐升高,而过氧化物酶(POD)活性响应晚于CAT;CAT在胁迫前中期起保护作用,而POD主要在中后期起保护作用。低温胁迫下,超氧阴离子和过氧化氢都迅速升高,在保护酶系的作用下,活性氧含量呈现动态变化,随着胁迫时间延长,后期超氧阴离子含量升高,过氧化氢含量下降。相关分析表明烟草花芽分化时间与活性氧含量变化有显著相关关系,推测低温胁迫下,烟苗体内活性氧代谢平衡状态的变化可能参与烟草的成花转变,一定程度地促进花芽分化,具体机制有待进一步研究。
2013 on the metabolism of reactive oxygen species in tobacco seedlings under low temperature stress and the relationship between reactive oxygen species and flower bud differentiation of tobacco. The results showed that under low temperature stress, the superoxide dismutase (SOD) of protective enzyme had the most rapid response, then the activity of catalase (CAT) increased gradually, while the activity of peroxidase (POD) Pre-stress from the protective effect, while the POD mainly in the late protective effect. Under the condition of low temperature stress, the superoxide anion and hydrogen peroxide rapidly increased. Under the action of the protective enzyme system, the active oxygen content showed a dynamic change. With the prolongation of stress time, the content of superoxide anion increased and the content of hydrogen peroxide decreased . Correlation analysis showed that there was a significant correlation between the time of flower bud differentiation and the change of reactive oxygen species. It is speculated that the changes of the equilibrium state of active oxygen metabolism in tobacco seedlings may be involved in the flower transformation of tobacco under low temperature stress, and promote the flower bud differentiation to a certain extent. further research.