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目的:研究β-榄香烯对体外培养的人涎腺腺样囊性癌SACC-83细胞中真核细胞翻译启始因子(eIF4E)蛋白表达的影响。方法:将SACC-83细胞分组培养,榄香烯1～4组培养液中分别加入200μL终浓度为60、80、100、120 mg/L的榄香烯注射液,空白对照组不加任何药物,阳性对照组加入顺铂(终浓度为3 mg/L)。培养12、24、48 h,光镜下观察细胞形态,采用免疫细胞化学方法检测细胞中eIF4E蛋白。结果:空白对照组SACC-83细胞生长、贴壁正常,胞质饱满均质透明,核浆比例大;榄香烯1～4组SACC-83细胞贴壁能力下降,体积变小变圆,胞膜皱褶,核浆比例减小,核边聚伴有碎裂。与空白对照组比较,榄香烯各组SACC-83细胞eIF4E蛋白表达水平较低(P均<0.05)。榄香烯1～4组SACC-83细胞eIF4E蛋白表达水平依次降低(P均<0.05),且随培养时间的延长eIF4E蛋白表达水平降低(P均<0.05)。结论:β-榄香烯可能通过下调SACC-83细胞eIF蛋白的表达,促进细胞凋亡,从而抑制其生长。
OBJECTIVE: To study the effect of β-elemene on the expression of eIF4E protein in human salivary adenoid cystic carcinoma SACC-83 cells in vitro. Methods: SACC-83 cells were cultured in groups. Elemene (200, 80, 100 and 120 mg / L) was injected into the elemene in culture medium of 1 to 4 groups respectively. The blank control group was given no drug , Positive control group added cisplatin (final concentration of 3 mg / L). The cells were cultured for 12, 24 and 48 hours. The morphology of cells was observed under light microscope. The eIF4E protein was detected by immunocytochemistry. Results: SACC-83 cells in the blank control group grew normally and adhered to each other. The cytoplasm of the SACC-83 cells were homogeneous and transparent with a large proportion of nuclear plasma. Membrane folds, the proportion of nuclear plasma decreases, the nucleus together with fragmentation. Compared with the blank control group, the expression of eIF4E protein in SACC-83 cells in all groups was lower (all P <0.05). The expression levels of eIF4E protein in SACC-83 cells were decreased in elemene group 1 ~ 4 (all P <0.05), and the expression of eIF4E protein decreased with the prolongation of culture time (all P <0.05). Conclusion: β-elemene may inhibit the growth of SACC-83 cells by down-regulating the expression of eIF protein and promoting cell apoptosis.