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为获知水环境中亚致死剂量的氯吡嘧磺隆对斑马鱼肝脏的影响,将斑马鱼暴露在亚致死剂量的氯吡嘧磺隆药液中8 d,采用超高效液相色谱与四级杆-轨道离子阱质谱串联对斑马鱼的肝脏代谢物进行分析,采用SIEVE软件将图谱信息转换为含有保留时间、质荷比和相对峰面积的二维数据阵.同时,结合mz Cloud、Human Metabolome Database(HMDB)数据库共鉴定出33种代谢物,包括氨基酸、糖、有机酸等物质.最后,采用SIMCA软件对正离子和负离子模式下的检测结果进行主成分、偏最小二乘法和正交校正的偏最小二乘法分析.主成分和偏最小二乘法结果表明,对照组与处理组在2种检测模式下均存在差异代谢物.为了寻找造成对照组与处理组之间差异的代谢物,对代谢物进行S-Plot分析,结果表明,肌酸酐、牛磺酸、多巴胺和5b-胆甾醇硫酸酯组间差异最大(p<0.01).4种代谢产物可作为斑马鱼在氯吡嘧磺隆亚致死剂量胁迫下肝脏代谢异常的潜在标记物.
In order to understand the influence of the low dose of trimethoprim-sulfamethoxazole on the liver of zebrafish in aquatic environment, the zebrafish was exposed to the sublethal dose of cyproterone for 8 days. The ultra-high performance liquid chromatography Rod-track ion trap mass spectrometry tandem analysis of zebrafish liver metabolites, the use of SIEVE software will map information is converted to a retention time, mass-to-charge ratio and relative peak area of two-dimensional data array.Meanwhile, combined with mz Cloud, Human Metabolome A total of 33 metabolites, including amino acids, sugars and organic acids, were identified in the database of Database (HMDB). Finally, the principal components, partial least squares and quadrature calibration of SIMCA were used to detect the positive and negative ions. Partial least squares analysis.The results of principal component analysis and partial least squares show that the control group and the treatment group have different metabolites in the two detection modes.In order to find the metabolites that cause the difference between the control group and the treatment group, S-Plot analysis of the metabolites showed that creatinine, taurine, dopamine and 5b-cholesterol sulfate groups had the greatest difference (p <0.01), and the four metabolites could be used as zebrafish in clopidogrel Sulfo lethal dose Longya stress potential hepatic metabolism markers.