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目的：探讨ｈＴＲ与肝细胞癌发生的关系，及与肝炎病毒感染的关系，扩大小样本检查在临床的应用，探讨端粒酶ｈＴＲ在肝细胞癌活检组织中的活性，并与手术切除的肝癌组织进行了比较。方法：６２例肝组织标本，其中４２例肝脏活检穿刺标本（１６例肝细胞癌，１４例肝硬化，１２例慢性肝炎），１８例手术切除的肝癌标本，２例尸解正常肝脏组织，均做了ｈＴＲ的活性检测。结果：外科手术切除的癌组织中，ｈＴＲ活性表达７２．２％（１３／１８），在针吸活检肝组织中，ｈＴＲ活性表达８１．３％（１３／１６），在高分化癌中７７．８％（７／９），中分化癌中９１．６７％（１１／１２），低分化癌中６２％（８／１３），在慢性肝炎、肝硬化中５３．８％（１４／２６）。６０例标本中有２６例病毒阳性标记，其中在病毒阳性及阴性标记的肝癌组织中ｈＴＲ阳性表达均为８３．３％（１０／１２），在病毒标记的慢性肝炎、肝硬化中ｈＴＲ活性表达５０％（７／１４），在肝癌旁组织中ｈＴＲ阳性表达８．３％（２／２４）。结论：端粒酶ｈＴＲ阳性表达与肝细胞癌的发生有关，与肝炎病毒标记状态无关，针吸活检标本在临床慢性肝脏疾病中，在小的不同类型结节性损伤的诊断中，端粒酶的分析是可行的。
Objective: To investigate the relationship between hTR and the occurrence of hepatocellular carcinoma, and its relationship with hepatitis virus infection. To expand the application of small sample examination in clinical practice. To investigate the activity of hTR in hepatocellular carcinoma biopsy and to compare with the surgical resection of liver cancer. The organization compared. Methods: 62 specimens of liver tissue, including 42 liver biopsy specimens (16 hepatocellular carcinomas, 14 cirrhosis, 12 chronic hepatitis), 18 surgically resected specimens of liver cancer, and 2 normal liver tissues autopsy The activity of hTR was tested. Results: hTR activity was expressed in 72.2% (13/18) of the surgically resected cancer tissues, and 81.3% (13/16) of the hTR activity was detected in the needle biopsy liver tissue. .8% (7/9), 91.67% (11/12) in moderately differentiated cancer, 62% (8/13) in poorly differentiated cancer, and 53.8% (14/26) in chronic hepatitis and cirrhosis ). There were 26 virus-positive markers in 60 specimens. The positive expression of hTR was 83.3% (10/12) in the virus-positive and negative-labeled hepatocellular carcinoma tissues. The expression of hTR activity in virus-labeled chronic hepatitis and liver cirrhosis. At 50% (7/14), hTR expression was 8.3% (2/24) positive in HCC tissues. Conclusion: The positive expression of telomerase hTR is associated with the occurrence of hepatocellular carcinoma, and has nothing to do with the status of the hepatitis virus marker. Needle biopsy specimens in clinical chronic liver disease, in the diagnosis of small different types of nodular injury, telomerase The analysis is feasible.