论文部分内容阅读
以极东锦鸡儿未成熟合子胚子叶为外植体进行其体细胞胚胎发生和植株再生研究。在添加不同BA与NAA或2,4-D,外加500mg·L~(-1)水解酪蛋白、30g·L~(-1)蔗糖和8g·L~(-1)琼脂的MS培养基上诱导产生了体细胞胚。在5mg·L~(-1)NAA+5mg·L~(-1)BA和5mg·L~(-1)2,4-D+1mg·L~(-1)BA处理中体胚诱导率分别为14%和10%;NAA处理每外植体上诱导出的体胚数量最多为4.3个,而2,4-D为10.5个。体细胞胚经成熟培养后,在添加0.01mg·L~(-1)NAA、20g·L~(-1)蔗糖和6g·L~(-1)琼脂的MS培养基上萌发率达到58.94%。萌发的体胚在MS培养基上长成正常小植株,再生率为87%。经炼苗后的体胚苗移植到草炭土:蛭石:珍珠岩=5:4:1(V/V/V)的栽培基质中,可以正常生长,移栽成活率为40%。
The study on somatic embryogenesis and plant regeneration of immature cotyledon embryo cotyledons of Caragana microphylla as explant. MS medium supplemented with BA and NAA or 2,4-D supplemented with 500 mg · L -1 hydrolyzed casein, 30 g · L -1 sucrose and 8 g · L -1 agar Induced production of somatic embryos. The somatic embryo induction rate in 5 mg · L -1 NAA + 5 mg · L -1 BA and 5 mg · L -1 2,4-D + 1 mg · L -1 BA treatment 14% and 10% respectively. NAA treatment induced a maximum of 4.3 somatic embryos per explant, while the 2,4-D was 10.5. After the somatic embryos were matured, the germination rate reached 58.94% on MS medium supplemented with 0.01 mg · L -1 NAA, 20 g · L -1 sucrose and 6 g · L -1 agar. . Germinating somatic embryos grow into normal plantlets on MS medium with a regeneration rate of 87%. The harvested somatic embryos were transplanted into peat soil: vermiculite: perlite = 5: 4: 1 (V / V / V) cultivation medium, which can grow normally. The survival rate after transplanting is 40%.