[Objective] To reveal the sterility genetic mode in Platycodon grandiflorum (Jacq.) A.DC, thus laying foundation for the utilization of male sterile materials. [Method] Hybridization, back-cross and selfing were performed between male sterile and normal germplasms for P. grandiflorum for observing the segregation of progenies. [Results] In F1 progenies of male sterile × normal fertile hybridization, there exist three combinations of fertility: (1) completely sterile, (2) completely fertile, and mostly (3) the segregating combination. The back-crossed progenies of completely sterile F1 combination still remained completely sterile. The selfed progenies of completely fertile F1 combination showed fertility segregation among individuals. Both the back-crossed and selfed progenies of the segregating F1 combination still presented fertility segregation among individuals. [Conclusion] According to the genetic features of nuclear and cytoplasmic nuclear infertility, the male sterile germplasms of P. grandiflorum detected in this study are infertile types of nuclear-cytoplasmic interaction, which can be used to produce three-line hybrid seeds using the conventional genetic breeding method. [Objective] To reveal the sterility genetic mode in Platycodon grandiflorum (Jacq.) A. DC, and laying foundation for the utilization of male sterile materials. [Method] Hybridization, back-cross and selfing were performed between male sterile and normal germplasms for P. grandiflorum for observing the segregation of progenies. [Results] In F1 progenies of male sterile × normal fertile hybridization, there exist three combinations of fertility: (1) completely sterile, (2) completely fertile, and mostly (3) the segregating combination. The back-crossed progenies of completely sterile F1 combination still remained completely sterile. The selfed progenies of completely fertile F1 combination showed fertility segregation among individuals. Both the back-crossed and selfed progenies of the segregating F1 combination still presented fertility segregation of individuals [Conclusion] According to the genetic features of nuclear and cytoplasmic nuclear infertility, the male sterile germplas ms of P. grandiflorum detected in this study are infertile types of nuclear-cytoplasmic interaction, which can be used to produce three-line hybrid seeds using the conventional genetic breeding method.