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目的:检测组织因子(TF)、尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)mRNA在肝细胞癌组织中的表达并探讨其临床意义。方法:利用RT-PCR法分别检测27例肝细胞癌、癌旁及27例正常肝组织中TF、uPA、uPAmRNA表达阳性率及相对表达强度,并结合临床病理资料进行分析。结果:TF、uPA、uPAR在肝细胞癌组织中阳性率及相对表达强度分别为62.96%(17/27)、70.37%(19/27)、77.78%(21/27);0.567±0.268、0.964±0.458、0.784±0.322,均显著高于癌旁组织及正常组织,差异显著(P<0.05)。3者在肝癌组织中相对表达强度与肿瘤大小及部分侵袭转移指标有关,其中TFmRNA表达强度在肝内及肝外转移及门脉癌栓组高于无肝内及肝外转移及无门脉癌栓组(P<0.05),uPAmRNA在有包膜侵润、肝内转移及门脉癌栓组高于无包膜侵润、无肝内转移及门脉癌栓组(P<0.05);uPARmRNA在有肝内转移及门脉癌栓组高于无肝内转移及门脉癌栓组(P<0.05)。经Pearson检验肝细胞癌患者TF、uPA和uPARmRNA表达呈正相关[TF-uPA:r=0.373(P<0.01),TF-uPAR:r=0.534(P<0.01),uPA-uPAR:r=0.365(P<0.01)]。结论:肝细胞癌组织中TF、uPA及uPAR的mRNA显著升高并与部分侵袭转移指标有关,提示3者可能在肝细胞癌的发生及侵袭转移起协同作用。
Objective: To detect the expression of tissue factor (TF), urokinase-type plasminogen activator (uPA) and its receptor (uPAR) mRNA in hepatocellular carcinoma (HCC) and to investigate its clinical significance. Methods: The positive rate and the relative expression intensity of TF, uPA and uPA mRNA in 27 cases of hepatocellular carcinoma, adjacent normal tissues and 27 cases of normal liver tissues were detected by RT-PCR, and analyzed with clinicopathological data. Results: The positive rate and the relative expression intensity of TF, uPA and uPAR in hepatocellular carcinoma were 62.96% (17/27), 70.37% (19/27), 77.78% (21/27), 0.567 ± 0.268 and 0.964 ± 0.458, 0.784 ± 0.322, respectively, which were significantly higher than those in paracancerous tissues and normal tissues (P <0.05). The relative expression intensity of 3 in HCC was correlated with tumor size and part of the invasion and metastasis index. The expression intensity of TF mRNA in intrahepatic and extrahepatic metastasis and portal vein thrombosis group were higher than those without intrahepatic and extrahepatic metastasis and without portal vein cancer (P <0.05). The expression of uPA mRNA in urokinase group was higher than that without urokinase invasion, intrahepatic metastasis and portal vein thrombosis (P <0.05) There was a higher incidence of intrahepatic metastasis and portal vein thrombosis than those without intrahepatic metastasis and portal vein tumor thrombosis (P <0.05). The levels of TF, uPA and uPAR mRNA were positively correlated with Pearson’s test in hepatocellular carcinoma patients (TF-uPA: r = 0.373, P <0.01), uPA-uPAR: r = 0.365 P <0.01)]. Conclusion: The mRNA levels of TF, uPA and uPAR in hepatocellular carcinoma were significantly increased and were related to some indicators of invasion and metastasis, suggesting that the three may play a synergistic role in the occurrence and invasion of hepatocellular carcinoma.