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目的:建立高效液相色谱法测定七香胃舒胶囊中黄芩苷的含量。方法:采用Agilent Eclipse Plus-C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相:甲醇-0.2%磷酸(47:53);流速:0.6 m·min~(-1);检测波长280 nm。结果:黄芩苷在0.083 4~0.834 0μg范围内线性关系良好(r=0.999 9),平均回收率为98.61%,RSD=0.86%(n=6)。结论:方法简便,灵敏、准确,能有效控制七香胃舒胶囊的质量。
Objective: To establish a method for the determination of baicalin in Qiuxuetouchu capsule by high performance liquid chromatography. Methods: The mobile phase was methanol-0.2% phosphoric acid (47:53), the flow rate was 0.6 m · min -1 using Agilent Eclipse Plus-C 18 (250 mm × 4.6 mm, 5 μm) Wavelength 280 nm. Results: The linearity of baicalin in the range of 0.083 4-0.834 0 μg was good (r = 0.999 9). The average recovery was 98.61% and the RSD was 0.86% (n = 6). Conclusion: The method is simple, sensitive and accurate, and can effectively control the quality of Qiuxuetouchu capsule.