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以铁皮石斛组培苗为试材,以松树皮和桑杆为基质,采用盆栽方式种植铁皮石斛组培苗,研究盆栽对铁皮石斛组培苗生长的影响及其不同生长阶段抗氧化生理特性的变化。结果表明:2种不同基质盆栽铁皮石斛组培苗在不同生长阶段叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和脯氨酸含量均显著高于移栽前,丙二醛(MDA)含量稍高于移栽前,叶绿素含量较移栽前的低,且2种不同基质的铁皮石斛叶片POD、SOD、CAT活性差异明显,脯氨酸含量、MDA含量和叶绿素含量差异不明显;2种不同基质盆栽铁皮石斛组培苗成活率都达90%以上,且移栽后长势好,移栽后2年株高分别为56.18、61.42cm,与大棚苗床栽培的效果比较接近。
Tissue culture seedlings of Dendrobium candidum were used as test materials, pine bark and mulberry rod were used as substrate, and potted plants were used to plant tissue culture of Dendrobium candidum. The effects of potted plants on tissue culture seedlings growth of Dendrobium officinale were studied, as well as the antioxidant physiological characteristics at different growth stages Variety. The results showed that the activities of POD, SOD, CAT and proline in leaves of two Dendrobium officinale plants were significantly different at different growth stages Higher than before transplanting, malondialdehyde (MDA) content slightly higher than before transplanting, chlorophyll content lower than before transplanting, and two kinds of matrix Dendrobium Dendrobium leaf POD, SOD, CAT activity significantly different, proline The contents of MDA, chlorophyll and chlorophyll content were not significantly different. The survival rates of two tissue culture plants of Dendrobium candidum were both higher than 90% after transplanting. The transplants showed good growth after transplanting. The plant height at two years after transplanting were 56.18 and 61.42 cm, And nursery greenhouse cultivation effect is relatively close.