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目的通过建立力竭性游泳运动损伤模型,探讨牡蛎多糖对力竭小鼠肝损伤的保护作用。方法实验小鼠30只,分为安静对照组、运动训练组、运动给药组,每组10只。运动给药组小鼠灌服剂量为100 g/kg/d牡蛎多糖溶液,其他两小组按质量灌服相应体积的生理盐水,共20 d。运动训练组、运动给药组隔天进行一次力竭游泳训练,末次运动后,测定各组小鼠血清ALT、AST,肝组织中MDA、GSH-PX、SOD、CAT、NO、NOS、Na+/K+-ATP、Ca2+/Mg2+-ATP。结果与安静对照组相比,运动训练组ALT、AST、MDA、NO、NOS升高(P<0.05、P<0.01),GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP降低(P<0.05、P<0.01);与运动训练组相比,运动给药组ALT、AST、MDA、NO、NOS降低(P<0.05、P<0.01),GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP均升高(P<0.05、P<0.01)。结论牡蛎多糖可以增强肝组织中抗氧化能力,抑制NOS的活性,减少的NO的毒性代谢物对肝组织的损伤。保持ATPase的活性。提示牡蛎多糖对力竭运动引起的肝损伤有一定的保护作用。
Objective To establish an exhaustive swimming injury model to investigate the protective effect of oyster polysaccharides on liver injury in exhausted mice. Methods Thirty mice were divided into four groups: control group, exercise training group and exercise group, with 10 rats in each group. The mice in the exercise group were dosed orally with 100 g / kg / d oyster polysaccharide solution, and the other two groups were given the same volume of saline by mass for 20 d. Exercise training group and exercise group were given exhaustive swimming training every other day. After the last exercise, serum ALT and AST, MDA, GSH-PX, SOD, CAT, NO, NOS and Na + K + -ATP, Ca2 + / Mg2 + -ATP. Results Compared with the quiet control group, the activities of ALT, AST, MDA, NO and NOS in the exercise training group were significantly increased (P <0.05, P <0.01) (P <0.05, P <0.01). Compared with the exercise training group, the activities of ALT, AST, MDA, NO and NOS in the exercise group decreased , Na + / K + -ATP, Ca2 + / Mg2 + -ATP increased (P <0.05, P <0.01). Conclusion Oyster polysaccharides can enhance the antioxidant capacity of liver tissue, inhibit the activity of NOS and reduce the toxic metabolites of NO on liver tissue damage. Maintain ATPase activity. Suggest oyster polysaccharides on liver injury caused by exhaustive exercise have a protective effect.