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目的研究饮食、运动和罗格列酮干预对肥胖大鼠心脏间质重构的影响。方法将SD大鼠随机分为正常对照组和高脂喂养组,后者在第8周时选取体质量超出正常对照组平均体质量20%者为肥胖大鼠,再分为肥胖对照组、单纯饮食干预组、饮食加运动干预组和饮食加罗格列酮组。实验第16周时测量心脏结构,检测血脂、血清纤溶酶原激活物抑制物-1(PAI-1)、心肌胶原含量及PAI-1基因的表达。结果高脂饮食性肥胖大鼠左心室肥厚,心肌胶原含量显著增加(P<0.05),PAI-1血清水平和mRNA表达显著增强(P<0.01)。单纯饮食干预组心室肥厚减轻,PAI-1mRNA表达下降(P<0.01)。运动干预组大鼠PAI-1表达明显降低(P<0.01),但左心室肥厚程度加剧。罗格列酮组胶原含量、PAI-1血清和心肌表达明显降低(P<0.05,P<0.01),且心脏结构明显改善。结论高脂饮食性肥胖大鼠心肌间质重构可能与PAI-1表达增强有关。调整饮食、有氧运动和罗格列酮干预均可不同程度的改善心肌重构。
Objective To investigate the effects of diet, exercise and rosiglitazone on cardiac interstitial remodeling in obese rats. Methods SD rats were randomly divided into normal control group and high-fat diet group, which at the eighth week the body weight of more than 20% of the normal control group average body weight of obese rats, and then divided into obese control group, simple Diet intervention group, diet plus exercise intervention group and diet plus rosiglitazone group. Cardiac structure was measured at the 16th week of the experiment. Serum lipids, serum plasminogen activator inhibitor-1 (PAI-1), cardiac collagen content and PAI-1 gene expression were measured. Results The left ventricular hypertrophy and myocardial collagen content were significantly increased in high-fat diet-induced obese rats (P <0.05). The serum and mRNA levels of PAI-1 were significantly increased (P <0.01). The ventricular hypertrophy was decreased and the expression of PAI-1 mRNA was decreased in the diet-only intervention group (P <0.01). PAI-1 expression in exercise intervention group was significantly lower (P <0.01), but left ventricular hypertrophy increased. Rosiglitazone group collagen content, PAI-1 serum and myocardial expression was significantly reduced (P <0.05, P <0.01), and significantly improved cardiac structure. Conclusions Myocardial interstitial remodeling may be related to the enhanced expression of PAI-1 in high-fat diet-induced obese rats. Adjusting diet, aerobic exercise and rosiglitazone intervention can improve myocardial remodeling in varying degrees.