论文部分内容阅读
本文研究了Bowman-Cole土壤有机磷分组法应用于石灰性土壤存在的若干问题,并提出了改进法。改进法的四组土壤有机磷(P0)的分组为:在氯仿预处理土样后用0.5molL-1NaHCO3浸提活性P0;然后,先用0.05molL-1NaOH、后用1molL-1H2SO4处理残余土样浸提中度活性P0,0.05molL-1NaOH提取的无机磷和1molL-1H2SO4提取的有机磷属于中度活性P0;在氯仿和0.5molL-1NaHCO3处理后,用0.05molL-1NaOH浸提稳定性P0,把浸提液的酸度调至pH3.00时,上清液中的有机磷为中度稳定性P0,而沉淀中的有机磷为高稳性P0。结果表明,改进法能更好地区分具有不同矿化率的有机磷化合物,例如,核酸、甘油磷酸、植酸钙、肌醇三磷酸铁、植酸铁等。改进法增加了超声波处理,使震荡时间也明显缩短。因此,石灰性土壤有机磷分组的改进法比Bowman-Cole法更理想。
In this paper, some problems about the application of Bowman-Cole soil organic phosphorus grouping method to calcareous soil were studied and the improved method was proposed. The four groups of soil organic phosphorus (P0) were grouped as follows: the active PO was extracted with 0.5molL-1NaHCO3 after pretreatment with chloroform; and then the residual soil samples were treated with 0.05molL-1NaOH and 1molL-1H2SO4 Extract moderate activity P0, 0.05molL-1NaOH extracted inorganic phosphorus and 1molL-1H2SO4 extracted organic phosphorus is moderate activity P0; chloroform and 0.5molL-1NaHCO3 treatment, with 0.05molL-1NaOH stability P0, When the acidity of the extract was adjusted to pH3.00, the organic phosphorus in the supernatant was moderately stable P0 while the organic phosphorus in the precipitate was highly stable P0. The results show that the improved method can better distinguish organophosphorus compounds with different mineralization rates, such as nucleic acid, glycerophosphate, phytin, inositol triphosphate and ferric phytate. Improved method to increase the ultrasonic treatment, the shock time is also significantly reduced. Therefore, the improved method of grouping organic phosphorus in calcareous soil is more ideal than the Bowman-Cole method.