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Lignosulfonate with low polydispersity index of 1.178-1.210 was isolated by gel column chromatography of Sephacryl S-100eluted with 0.2 mol/L of NaNO3 aqueous solution,whereas nearly monodisperse ligosulfonate fraction with polydispersity of 1.067can be obtained after chromatographic separation twice.This method provides an available approach to investigate the structure and characteristics of lignosulfonate.