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用抗阴道毛滴虫的单克隆抗体ＩｇＧ（鼠）和兔抗阴道毛滴虫多克隆抗体进行ＥＬＩＳＡ试验（双抗体夹心法），检测阴道毛滴虫可溶性抗原。分别观察了单克隆和多克隆抗体的不同浓度、不同的封闭剂型、封闭时间及孵育时间等条件对本试验效果的影响。结果以单克隆抗体ＩｇＧ（鼠）２５μｇ／ｍｌ包被，用０．２５％脱脂奶粉１次性封闭０．５ｈ（３７℃），兔抗阴道毛滴虫多克隆抗体滴度１∶２００，孵育０．５ｈ为最佳条件。阴道毛滴虫可溶性抗原检出最低量为０．３μｇ。本试验与多种寄生虫的多克隆抗体均无交叉反应
The anti-Trichomonas vaginalis soluble antigens were detected by ELISA (double-antibody sandwich method) with monoclonal antibody against Trichomonas vaginalis IgG (mouse) and rabbit anti-Trichomonas vaginalis polyclonal antibody. The effects of different concentrations of monoclonal and polyclonal antibodies, different blocking agents, blocking time and incubation time on the effect of this test were observed. The results were coated with monoclonal antibody IgG (mouse) 25μg / ml, blocked with 0.25% non-fat dry milk 0.5h (37 ℃), rabbit polyclonal antibody Trichomonas vaginalis titers 1: 200, incubated 0.5h is the best condition. Trichomonas vaginalis soluble antigen detection of the minimum amount of 0.3μg. This test and a variety of parasite polyclonal antibodies were not cross-reaction