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小胶质细胞制片的难度比较大,不易成功;我经过摸索改良,终于取得较好的效果。我所用方法基本上是翻译国外的Hortega方法,但是完全按他的方法做,底色不好,沉淀多,小胶质数量少。经过改良,克服了上述缺点,本文着重介绍改良之处。1.取材:成年兔大脑切2—5mm,室温最好22℃—25℃。2.固定:用福尔马林溴化铵(FAB)固定液固定,4—8天均好,时间愈长效果愈好,可据需要选择。冰冻切片前,将脑组织放入新鲜FAB固定液里,加温至49℃—50℃,保温10分钟,换入蒸馏水,如FAB固定液内冷却效果更好。
The difficulty of microglial cell preparation is relatively large, not easy to succeed; I improved after groping and finally achieved better results. The method I used was basically to translate the Hortega method abroad, but it was done exactly as he did. It had a poor base color, a lot of sedimentation and a small amount of microgolls. After the improvement, to overcome the above shortcomings, this article focuses on the improvements. 1. Draws: Adult rabbit brain cut 2-5mm, the best room temperature 22 ℃ -25 ℃. 2. Fixed: fixed with formalin ammonium bromide (FAB) fixative, 4-8 days are good, the longer the better the effect, according to need to choose. Frozen sections, the brain tissue into fresh FAB fixative, warmed to 49 ℃ -50 ℃, incubated for 10 minutes, into distilled water, such as FAB solution cooling better.