论文部分内容阅读
本实验用成年雄性Wistar大鼠53只,分为溃疡组、盐水对照组和正常对照组。在手术后4、10,14、21及28天分批取材。用Sternberger PAP法进行免疫组织化学染色,分别显示胃窦粘膜胃泌素细胞(G细胞)和生长抑素细胞(D细胞),观察大鼠实验性胃溃疡自愈期间,G和D细胞的形态变化,并进行了细胞计数和统计学分析。本文结果表示,溃疡组G细胞数量在术后10至14天明显增多(P<0.01),21、28天趋于减少,但仍高于对照组。有些G细胞排列成群,密集呈明显带状。正常时G细胞分布在幽门腺的中、下1/3处,而溃疡组可见有些G细胞分布在腺的上部。D细胞数量仅在术后10天增多(P<0.01),与正常对照组相比差异显著。用免疫组织化学双重染色法,可见G细胞和D细胞之间存在着形态上的接触。溃疡自愈期间,G/D细胞比值未见明显改变。本实验结果提示,胃窦粘膜局部G、D细胞的变化和大鼠实验性胃溃疡自愈修复之间有着一定的联系。
In this experiment, 53 adult male Wistar rats were divided into ulcer group, saline control group and normal control group. Materials were taken in batches on days 4, 10, 14, 21 and 28 after surgery. Sternberger PAP was used for immunohistochemical staining to show gastrin (Gas) cells and somatostatin (D) cells in gastric antrum. The morphology of G and D cells during the healing of experimental gastric ulcers in rats was observed. Changes, and conducted cell counts and statistical analysis. The results of this study indicate that the number of G cells in the ulcer group increased significantly (P<0.01) from day 10 to day 14 after surgery, and decreased on days 21 and 28, but it was still higher than that of the control group. Some G cells line up in clusters and are densely banded. Normally, G cells are distributed in the middle and lower 1/3 of the pyloric gland, while in the ulcer group, some G cells are distributed in the upper part of the gland. The number of D cells increased only 10 days after surgery (P<0.01), and there was a significant difference compared with the normal control group. With immunohistochemical double staining, there was a morphological contact between G cells and D cells. There was no significant change in the ratio of G/D cells during ulcer healing. The results of this experiment suggest that the changes of local G and D cells in gastric antrum mucosa are related to the repair of experimental gastric ulcer in rats.