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大鼠经20h电刺激应激反应后,腹腔巨噬细胞释放H_2O_2量明显减少(P<0.001),双侧肾上腺摘除术可以对抗应激对巨噬细胞释放H_2O_2功能的抑制作用(P<0.001),而单纯摘除大鼠双侧肾上腺对巨噬细胞无明显影响。将与肾上腺有关的激素与巨噬细胞体外孵育20h后发现,去甲肾上腺素和肾上腺素对巨噬细胞无明影响;氢化可的松可以明显促进巨噬细胞释放H_2O_2功能;促肾上腺皮质激素可以明显抑制巨噬细胞释放H_2O_2功能(P<0.01)。大鼠在应激反应后血浆皮质酮含量明显升高(P<0.05)进一步观察氢化可的松任体内对巨噬细胞功能的影响发现,皮下注射氢化可的松可以明显抑制巨噬细胞释放H_2O_2功能(P<0.01)。以上结果提示应激对巨噬细胞释放H_2O_2具有抑制作用,这种抑制作用的产生与垂体-肾上腺皮质系统的凋节作用有关。
After 20h electrical stimulation, the amount of H_2O_2 in peritoneal macrophages was significantly decreased (P <0.001). Adrenalectomy could counteract the inhibitory effect of stress on the release of H_2O_2 by macrophages (P <0.001) , While the simple adrenalectomy rat macrophages no significant effect. After adventitial hormone and macrophage were incubated in vitro for 20h, norepinephrine and epinephrine had no obvious effect on macrophage. Hydrocortisone could obviously promote the release of H_2O_2 from macrophage. Adrenocorticotropic hormone could Obviously inhibited the release of H_2O_2 by macrophages (P <0.01). The level of plasma corticosterone was significantly increased after stress reaction in rats (P <0.05). The effect of hydrocortisone on the function of macrophages was further observed. Hydrocortisone subcutaneously inhibited the release of H 2 O 2 from macrophages (P <0.01). The above results suggest that stress can inhibit the release of H 2 O 2 from macrophages, and this inhibitory effect is related to the apoptosis of the pituitary-adrenal system.