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目的研究大肠癌Ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）和ＣＥＡ的表达与淋巴结转移的关系．方法应用链霉菌素生物素（ＳＰ）免疫组化法，观察４４例大肠癌Ｐ５３，ＰＣＮＡ的阳性率和ＣＥＡ的表达型式．结果大肠癌Ｐ５３阳性率为５２３％；大肠癌Ｐ５３阳性表达与性别、年龄及肿瘤的部位、分化程度和浸润深度无关（Ｐ＞００５）；大肠癌Ｐ５３阳性者其淋巴结转移率较阴性者高（１４／２３，６０９％ｖｓ６／２１，２８６％，Ｐ＜００５）；Ｐ５３阳性表达及有淋巴结转移者其细胞增殖活性分别较Ｐ５３阴性表达及无淋巴结转移者高（５５９±１７ｖｓ３７９±１４，Ｐ＜００５；５６２±１５ｖｓ３９６±１７，Ｐ＜００５）；Ｐ５３阳性表达及有淋巴结转移者其ＣＥＡ表型均以胞质型和间质型为主（２１／２３，９１３％ｖｓ１３／２１，６１９％，Ｐ＜００５；１９／２０，９５０％ｖｓ１５／２４，６２５％，Ｐ＜００５）．结论检测Ｐ５３和ＰＣＮＡ表达及ＣＥＡ表型对判断大肠癌的恶性程度，预测其淋巴结转移趋势和预后及指导临床治疗有重要价值．
4. Objective To study the relationship between the expression of P53 protein, proliferating cell nuclear antigen (PCNA) and CEA and lymph node metastasis in colorectal cancer. Methods Streptomycin-biotin (SP) immunohistochemistry was used to observe the positive rate of P53, PCNA and the expression of CEA in 44 cases of colorectal cancer. Results The positive rate of P53 in colorectal cancer was 52. 3%. The positive expression of P53 in colorectal cancer was not related to the sex, age, tumor location, differentiation and depth of invasion (P>005). The rate of lymph node metastasis in colorectal cancer was positive in P53. The negative patients were high (14/23, 609% vs6/21, 286%, P<005); P53 positive expression and lymph node metastasis were more active than P53 negative and no lymph node metastasis. High (559±17vs379±14, P<005;562±15vs396±17, P<005); positive expression of P53 and lymph node metastasis The CEA phenotypes were mainly cytoplasmic and interstitial (21/23, 913% vs13/21, 619%, P<005; 19/20, 950% vs15/ 24,625%, P<005). Conclusion The detection of P53 and PCNA expression and CEA phenotype have important value in judging the malignant degree of colorectal cancer, predicting its lymph node metastasis tendency and prognosis, and guiding clinical treatment.