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目的:研究Notch1在大鼠舌癌模型发生发展过程中的表达变化。方法:将40只SD大鼠随机分为对照组(A组10只)和实验组(B、C、D组各10只),使用质量百分比浓度为0.004%的4-硝基喹啉-1-氧化物(4-nitroquinoline-1-oxide,4NQO)饮用水喂养实验组SD大鼠,用蒸馏水喂养对照组SD大鼠。B、C、D组分别于8、16、24周取大鼠舌体组织;A组在8、16、24周同时各取3只、3只、4只作为正常对照。行常规组织学观察,并使用免疫组织化学法检测Notch1在病变不同阶段的舌粘膜内的表达。结果:4NQO可以有效诱导大鼠舌黏膜鳞状细胞癌的发生。在不同的给药时间点,可以诱导大鼠舌黏膜产生不同程度的癌前病变。Notch1在正常舌黏膜、癌前病变、舌鳞癌中的表达逐渐增高(P<0.01)。随着异常增生程度的增加,阳性表达部位逐渐由基底层向表层扩展,且Notch1的膜型表达逐渐增多。结论:Notch1的高表达及其在细胞膜上的集中表达可能与舌癌的发生、发展过程相关。
Objective: To investigate the expression changes of Notch1 in rat tongue cancer model. Methods: Forty SD rats were randomly divided into control group (10 rats in group A) and experimental group (10 rats in group B, C and D). The rats were treated with 4-nitroquinoline-1 (4-nitroquinoline-1-oxide, 4NQO) drinking water was fed to the SD rats in the experimental group, and the SD rats in the control group were fed with distilled water. The tongue tissues of rats in groups B, C and D were taken at 8, 16 and 24 weeks respectively. In group A, 3, 3 and 4 were taken as normal control at 8, 16 and 24 weeks respectively. Histopathological examination was performed and the expression of Notch1 in the tongue mucosa at different stages of the lesion was detected by immunohistochemistry. Results: 4NQO can effectively induce the occurrence of squamous cell carcinoma of tongue mucosa in rats. At different time points of administration, different degrees of precancerous lesions can be induced in rat tongue mucosa. Notch1 expression in normal tongue mucosa, precancerous lesions, tongue squamous cell carcinoma gradually increased (P <0.01). With the increase of the degree of abnormal proliferation, the positive expression sites gradually spread from the basal layer to the superficial layer, and the membrane type expression of Notch1 gradually increased. Conclusion: The high expression of Notch1 and its concentration on the cell membrane may be related to the occurrence and development of tongue cancer.