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新型缓释尿素的成功研制对提高肥料利用率、降低环境污染有重要意义。用模式植物拟南芥作为研究对象,普通尿素作为对照,通过拟南芥主根长、鲜重、须根数研究新型缓释尿素对拟南芥生长的影响。用控释剂对拟南芥施用,通过拟南芥同源重组频率研究控释剂对拟南芥遗传毒性的影响。结果表明,缓释尿素及普通尿素随着浓度增加对拟南芥的生长都有先促进后抑制的趋势,低浓度施用条件下缓释尿素对拟南芥的生长促进作用更加明显,高浓度施用条件下普通尿素对拟南芥的抑制作用更加明显。尿素浓度达到1000μg/mL时,拟南芥可以正常萌发,但是不能存活。控释剂施用对拟南芥遗传毒性分析表明,控释剂不影响拟南芥同源重组频率,表明在本实验浓度范围内控释剂暴露对拟南芥未产生遗传毒性影响。研究结果为该新型缓释尿素在生产上应用推广打下基础。
The successful development of a new type of slow-release urea is of great significance to improve fertilizer utilization and reduce environmental pollution. The Arabidopsis thaliana model was used as the research object and the common urea was used as a control to study the effects of the new slow release urea on the growth of Arabidopsis thaliana by using the main root length, fresh weight and number of fibrous roots of Arabidopsis thaliana. Arabidopsis was treated with controlled release agent and the effect of controlled release agent on genotoxicity in Arabidopsis thaliana was studied by homologous recombination frequency in Arabidopsis thaliana. The results showed that both slow-release urea and normal urea tended to first promote and then inhibit the growth of Arabidopsis thaliana with increasing concentration. Slow-release urea under the condition of low concentration had a more obvious effect on the growth promotion of Arabidopsis thaliana, Under the conditions of ordinary urea inhibition of Arabidopsis more obvious. When urea concentration reached 1000μg / mL, Arabidopsis could germinate normally, but could not survive. Genetic toxicity analysis of controlled release formulations in Arabidopsis showed that the controlled release agent did not affect Arabidopsis homologous recombination frequency, indicating that no release of the controlled release agent has a genotoxic effect on Arabidopsis thaliana at this concentration range. The research results lay the foundation for the application and promotion of the new slow release urea in production.