论文部分内容阅读
目的:比较浸透限制固定相高效液相色谱法(RAM-HPLC)与荧光偏振免疫法(FPIA)测定人血中的卡马西平(CBZ)浓度.方法:建立一种直接进样RAM-HPLC法测定病人静脉和指端血浆的CBZ浓度.结果:两种测定方法都不需样品前处理,有好的重现性和近100％的回收率.FPIA测定静脉血样CBZ的结果与RAM-HPLC分别测定静脉和指端血样CBZ的结果有良好的相关性(R=0.989,0.995),但相差显著(P<0.05);而RAM-HPLC法测定静脉和指端血样两组数据间相差不显著(P>0.05).结论:RAM-HPLC和FPIA法均可测定CBZ浓度.在治疗药物监测中,FPIA更适合常规的监测;RAM-HPLC法更适用于相关研究和特殊病例监测,这里我们成功地应用于测定人外周微量血样中CBZ的浓度.
OBJECTIVE: To determine the concentration of carbamazepine (CBZ) in human blood by RAM-HPLC and fluorescence polarization immunoassay (FPIA) .Methods: A direct injection RAM-HPLC method The concentration of CBZ in venous and finger plasma of patients was measured.Results: Both of the two methods were without sample pretreatment, with good reproducibility and recovery rate of nearly 100% There was a good correlation between the results of the determination of venous and finger-tip CBZ (R = 0.989, 0.995), but the difference was significant (P <0.05); while there was no significant difference between RAM- P> 0.05) .Conclusion: Both the RAM-HPLC and the FPIA assay can determine the concentration of CBZ, FPIA is more suitable for routine monitoring in therapeutic drug monitoring, RAM-HPLC method is more suitable for related research and special case monitoring, here we successfully Used to determine the concentration of CBZ in human peripheral micro-blood samples.