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目的 分析小儿急性白血病 (AL)的免疫表型、异倍体、细胞周期分布状况的临床意义。方法 应用流式细胞仪检测了 4 2例初诊为AL患儿的免疫表型及骨髓单个核细胞 (MNC)的DNA含量。结果 B -ALL明显多于T -ALL ,髓系抗原在ANLL组中的表达率依次为CD3 3 >CD13 >CD14>CD15 。 4例AL同时表达了淋系和髓系的抗原 ,预后差。AL患儿骨髓细胞周期的S期细胞比例 (S % )明显低于正常对照 ,说明AL患儿白血病细胞的增殖能力低下。ALL组中 ,亚倍体的S %均值与超二倍体、二倍体比较存在显著差异性 ,ANLL组中 ,CD3 3/CD13<1者的S %均值与CD3 3 /CD13 >1者比较亦存在显著差异。结论 AL患儿的免疫表型、倍体关系、细胞周期及其之间的关系 ,对临床诊断、治疗、预后判断具有重要指导意义。
Objective To analyze the clinical significance of immunophenotype, aneuploidy and cell cycle distribution in childhood acute leukemia (AL). Methods The immunophenotype and DNA content of bone marrow mononuclear cells (MNCs) of 42 newly diagnosed children with Al were detected by flow cytometry. Results B-ALL was significantly more than T -ALL, myeloid antigens in the ANLL group were CD3 3> CD13> CD14> CD15. 4 cases of AL expression of both lymphoid and myeloid antigens, the prognosis is poor. AL patients with bone marrow cell cycle S phase cell ratio (S%) was significantly lower than the normal control, indicating that AL children with leukemic cells proliferation is low. In ALL group, there was a significant difference in S% mean of diploid with diploid and diploid, in ANLL group, the mean S% of CD3 3 / CD13 <1 was compared with that of CD3 3 / CD13> 1 There are also significant differences. Conclusion The immunophenotypic, ploidy relationship, cell cycle and the relationship between them in AL children are of great guiding significance for clinical diagnosis, treatment and prognosis.