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目的:建立滇白珠中槲皮苷的含量测定方法。方法:采用高效液相色谱法,以Appollo-C18色谱柱为分离柱,以乙腈-0.1%磷酸(23∶77)为流动相,254 nm为检测波长,柱温为室温。结果:槲皮苷在0.027 5～0.88μg峰面积与其质量浓度呈良好线性关系(r=1.00),平均回收率为99.0%,RSD 2.37%(n=6)。结论:该方法简便、准确、重复性好,可作为该药材含量测定方法。
Objective: To establish a method for the determination of quercetin in Dianban Beidou. Methods: HPLC with Appollo-C18 column as mobile phase, acetonitrile-0.1% phosphoric acid (23:77) as mobile phase, 254 nm as detection wavelength and column temperature as room temperature. Results: The peak area of quercetin in the range of 0.027 5 ~ 0.88 μg was linear with its mass concentration (r = 1.00). The average recovery was 99.0% and the RSD was 2.37% (n = 6). Conclusion: The method is simple, accurate and reproducible, and can be used as a method for the determination of the content of the medicinal materials.