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目的评价内磁场在磁靶向治疗胆道恶性肿瘤中的作用。方法建立异位胆管癌移植瘤裸鼠模型32只,随机分成4组,每组8只。A组(实验组):采用自制的胆道磁性支架丝,在肿瘤内部建立30mT的磁场,尾静脉注射纳米氟尿嘧啶(5-FU)磁小体;B组(空白对照组):肿瘤模型自然生长,无磁场和药物应用;C组(单纯内磁场组):建立与A组一致的肿瘤局部内磁场,无药物治疗;D组(外磁场组):建立500mT的肿瘤局部外磁场,药物干预同A组。在首次治疗当天和治疗后第4、7、11、15、19天,分别测各组肿瘤体积,做重复测量的多因素方差分析。观察期结束后处死裸鼠,观察肿瘤组织病理变化。结果与空白对照组比较,单纯内磁场组、外磁场组和实验组的肿瘤抑制率依次为18.039%、26.078%、40.12%,实验组肿瘤生长受到明显抑制,与3个对照组的肿瘤体积均有显著性差异,该组肿瘤组织镜下显示大量细胞凋亡,可见大量纳米磁小体颗粒沉积在凋亡的肿瘤细胞内。结论基于内磁场的磁靶向治疗可抑制肿瘤生长,其效果优于传统的依靠外磁场的靶向治疗方法。
Objective To evaluate the role of magnetic field in magnetic targeting therapy of biliary malignancies. Methods 32 nude mice model of ectopic cholangiocarcinoma xenografts were established and randomly divided into 4 groups with 8 mice in each group. Group A (experimental group): A magnetic field of 30 mT was established inside the tumor by self-made biliary magnetic stent wire. Nano-fluorouracil (5-FU) magnetosomes were injected through the tail vein; Group B (blank control group) No magnetic field and drug application; Group C (purely internal magnetic field group): establishing the local magnetic field of the tumor consistent with Group A without drug treatment; Group D (external magnetic field group): establishing a 500 mT local magnetic field of the tumor, group. On the first day of treatment and on the 4th, 7th, 11th, 15th and 19th day after treatment, the tumor volume of each group was measured and the multivariate ANOVA of repeated measures was performed. After the observation period, the nude mice were killed and the histopathological changes of the tumor were observed. Results Compared with the blank control group, the tumor inhibition rates in the purely magnetic field group, the external magnetic field group and the experimental group were 18.039%, 26.078% and 40.12%, respectively. The tumor growth was significantly inhibited in the experimental group and the tumor volume in the three control groups There were significant differences in this group of tumor tissue microscopy showed a large number of apoptosis, showing a large number of nano-magnetosome particles deposited in apoptotic tumor cells. Conclusion Magnetic target therapy based on the magnetic field can inhibit the growth of the tumor, and its effect is superior to the traditional targeted therapy based on external magnetic field.