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本研究利用SRAP分子标记技术研究30份苦荬菜(Ixeris polycephala)间遗传关系和遗传多样性,从209对引物组合里筛选出32对条带清晰,多态性好的引物组合。扩增总条带数430条,多样性条带381条,多态性百分率88.60%;栽培驯化新品系平均遗传相似系数0.837,苦荬菜品种平均遗传相似性系数为0.805,POPGENE分析得知基因多样性指数变幅0.128 3~0.260 3,Shannon指数变幅0.189 8~0.394 3,其中栽培驯化新品系多态性条带、基因多样性指数、Shannon指数要略高于苦荬菜品种,说明栽培驯化新品系与苦荬菜品种有一定的相似性,其遗传多样性水平要略高于苦荬菜品种;UPMGA聚类分析将30份苦荬菜划分为6个类群,6个苦荬菜品种与18个栽培驯化新品系划分在第I类群,野生种被划分在其余5个类群中,聚类分析与主成分分析结果相对一致。基于SRAP标记分析不同类型间苦荬菜遗传关系,为牧草新品种选育优良杂交组合提供重要的理论依据。
In this study, SRAP molecular markers were used to study the genetic relationship and genetic diversity of 30 Ixeris polycephala cultivars. A total of 32 pairs of primer combinations with clear and polymorphic bands were selected from 209 primer combinations. The total number of amplified bands was 430 and the diversity bands were 381 with a percentage of 88.60%. The average genetic similarity coefficient of cultivated and domesticated new strains was 0.837, and the average genetic similarity coefficient of bittergourd cultivars was 0.805. POPGENE analysis showed that the genes Diversity index 0.128 3 ~ 0.260 3 Shannon index amplitude 0.189 8 ~ 0.394 3, which cultivated and domesticated new strains of polymorphic bands, genetic diversity index Shannon index slightly higher than bitter gourd varieties, indicating that cultivation and domestication The new line had some similarities with bitter gourd varieties, and the genetic diversity level was slightly higher than that of bitter gourd varieties. UPMGA clustering analysis divided 30 bitter gourd divisions into 6 groups, 6 bitter gourd varieties and 18 The cultivated and domesticated new lines were divided into the first group, the wild species were divided into the remaining five groups, the cluster analysis and the principal component analysis results are relatively consistent. Based on the SRAP markers, the genetic relationships among different types of bitter canulas were analyzed, which provided important theoretical basis for the selection and breeding of excellent new crossbred varieties.