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[Objective] This study aimed to investigate the antimicrobial activity of hybrid antimicrobial peptide buforin II-cecropin B. [Method] Gene fragment BC encoding the hybrid antimicrobial peptide Buforin II-Cecropin B was synthesized by SOE-PCR with six primers designed according to the published amino acid sequences. Then,the BC gene fragment was ligated into p ET32 a vector and expressed in BL21. Antimicrobial ability of the crude hybrid antimicrobial peptide was detected with the Oxford cup method. [Result]The BC gene fragment was correctly amplified by SOE-PCR and ligated into p ET32 a vector,to construct the expression vector p ET32-BC. The hybrid antimicrobial peptide buforin II-cecropin B was expressed in BL21 after induction by IPTG. The optimal induction time was 2 h.Antimicrobial activity assay suggested that the hybrid antimicrobial peptide possessed antimicrobial activity to Escherichia coli and Bacillus subtilis. [Conclusion]The hybrid antimicrobial peptide buforin II-cecropin B exhibited antimicrobial activity to Gram-negative E. coli and Gram-positive B. subtilis strains.
[Objective] This study aimed to investigate the antimicrobial activity of hybrid antimicrobial peptide buforin II-cecropin B. [Method] Gene fragment BC encoding the hybrid antimicrobial peptide Buforin II-Cecropin B was synthesized by SOE-PCR with six primers designed according to the published amino acid sequences. Then, the BC gene fragment was ligated into p ET32 a vector and expressed in BL21. Antimicrobial ability of the crude hybrid antimicrobial peptide was detected with the Oxford cup method. [Result] The BC gene fragment was correctly amplified by SOE-PCR and ligated into p ET32 a vector, to construct the expression vector p ET32-BC. The hybrid antimicrobial peptide buforin II-cecropin B was expressed in BL21 after induction by IPTG. The optimal induction time was 2 h. Antimicrobial activity assay suggested that the hybrid antimicrobial peptide possessed antimicrobial activity to Escherichia coli and Bacillus subtilis. [Conclusion] The hybrid antimicrobial peptide buforin II-cecrop in B exhibited antimicrobial activity to Gram-negative E. coli and Gram-positive B. subtilis strains.