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目的研究Ovol2基因对肺腺癌细胞上皮-间质转化(epithelial-mesenchymal transition,EMT)过程及其侵袭能力的影响,以期为肺腺癌的靶向治疗提供一定的理论基础。方法通过体外培养肺腺癌细胞株A549,并感染PLV-BSD-Ovol2及PLV-BSD-Control慢病毒形成实验组(过表达Ovol2)和对照组;通过实时荧光定量PCR实验(quantitative real-time PCR,Real-time PCR)检测与EMT相关基因的mRNA表达水平变化;用蛋白印迹法检测相关标记蛋白,如E-钙黏蛋白(E-cadherin)及波形蛋白(vimentin)的表达水平;划痕实验(wound healing)及侵袭实验(transwell)比较实验组和对照组细胞的转移和侵袭能力的变化。结果 Ovol2基因过表达后,不论在mRNA还是在蛋白表达水平,E-钙黏蛋白表达上升,N-钙黏蛋白及波形蛋白表达下降;同时肺癌细胞的转移和侵袭能力也相应被抑制。结论 Ovol2基因的过表达会通过抑制肺腺癌细胞的EMT过程来抑制其远处转移和侵袭力。
Objective To investigate the effect of Ovol2 gene on epithelial-mesenchymal transition (EMT) and invasion of lung adenocarcinoma cells in order to provide some theoretical basis for the targeted therapy of lung adenocarcinoma. Methods The lung adenocarcinoma cell line A549 was cultured and infected with PLV-BSD-Ovol2 and PLV-BSD-Control lentivirus to form experimental group (overexpression Ovol2) and control group. Real-time quantitative PCR Real-time PCR was used to detect the mRNA expression level of EMT related genes. Western blotting was used to detect the expression of related marker proteins such as E-cadherin and vimentin. (wound healing) and invasion (transwell) were compared between the experimental group and the control group cells in metastasis and invasion ability changes. Results After overexpression of Ovol2 gene, the expression of E-cadherin, N-cadherin and vimentin decreased in both mRNA and protein expression level. Meanwhile, the metastasis and invasive ability of Ovol2 gene were also inhibited. Conclusion Overexpression of Ovol2 inhibits the distant metastasis and invasiveness of lung adenocarcinoma cells by inhibiting the EMT process.